Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion-promoting activity
| Autor: | Motoharu Seiki, Takamasa Uekita, Ikuo Yana, Hiroshi Ohno, Yoshifumi Itoh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cytoplasm
media_common.quotation_subject Recombinant Fusion Proteins education CHO Cells Matrix metalloproteinase MT-MMP metalloproteinase internalization invasion migration Transfection Clathrin Article Extracellular matrix Invasion Cell Movement Cricetinae Animals Humans Internalization Metalloproteinase Migration media_common Tissue Inhibitor of Metalloproteinase-2 biology Transferrin Signal transducing adaptor protein Cell migration Clathrin-Coated Vesicles Cell Biology Matrix Metalloproteinases Recombinant Proteins Transport protein Cell biology Protein Transport Membrane protein biology.protein Metallothionein Matrix Metalloproteinase 1 |
| Zdroj: | The Journal of Cell Biology |
| Popis: | 金沢大学自然科学研究科 理化学研究所・横浜研究所 免疫アレルギー科学総合研究センター(RCAI) 横浜市立大学大学院国際総合科学研究科生体超分子科学専攻 客員教授 Membrane-type 1 matrix metalloproteinase (MT1-MMP) is an integral membrane proteinase that degrades the pericellular extracellular matrix (ECM) and is expressed in many migratory cells, including invasive cancer cells. MT1-MMP has been shown to localize at the migration edge and to promote cell migration; however, it is not clear how the enzyme is regulated during the migration process. Here, we report that MT1-MMP is internalized from the surface and that this event depends on the sequence of its cytoplasmic tail. Di-leucine (Leu571–572 and Leu578–579) and tyrosine573 residues are important for the internalization, and the µ2 subunit of adaptor protein 2, a component of clathrin-coated pits for membrane protein internalization, was found to bind to the LLY573 sequence. MT1-MMP was internalized predominantly at the adherent edge and was found to colocalize with clathrin-coated vesicles. The mutations that disturb internalization caused accumulation of the enzyme at the adherent edge, though the net proteolytic activity was not affected much. Interestingly, whereas expression of MT1-MMP enhances cell migration and invasion, the internalization-defective mutants failed to promote either activity. These data indicate that dynamic turnover of MT1-MMP at the migration edge by internalization is important for proper enzyme function during cell migration and invasion. |
| Databáze: | OpenAIRE |
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