Improved insulin receptor assay: effects of an antidiabetic sulphonylurea on liver membrane insulin receptors from obese hyperglycaemic mice
| Autor: | B.D. Greenstein |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Population Mice Obese Cell membrane Mice Internal medicine medicine Animals Hypoglycemic Agents Insulin education Receptor Incubation Pharmacology education.field_of_study biology Cell Membrane Receptor Insulin Insulin receptor Kinetics Endocrinology medicine.anatomical_structure Sulfonylurea Compounds Membrane protein Liver Hyperglycemia biology.protein Hormone Research Article |
| Zdroj: | British journal of pharmacology. 66(2) |
| ISSN: | 0007-1188 |
| Popis: | 1 A simple assay of liver membrane insulin receptors is described, under conditions which apparently stabilized the insulin-receptor complex. The receptor population was measured in the presence of physiological doses of radioactive hormone under conditions which minimized negative co-operativity effects. 2 Equilibrium between receptor and hormone was attained in vitro within 60 min of incubation time at 13 degrees C with no apparent loss of binding sites after 3 h of incubation. 3 Liver membranes from lean mice possessed 8.8 times the receptor number per mg membrane protein measured in membranes from obese hyperglycaemic (ob/ob) littermates. After treatment of ob/ob mice with ARDF-26 (Gliquidon; Boehringer Ingelheim Ltd) the concentration of liver membrane insulin receptors was increased 5 fold. |
| Databáze: | OpenAIRE |
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