The Effect of Erythritol on the Stability of γ-glutamyl Transpeptidase and N-acetyl Glucosaminidase in Human Urine
| Autor: | W F Loeb, S R Das, J R Trout |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Time Factors 040301 veterinary sciences Urine Erythritol Toxicology 030226 pharmacology & pharmacy Pathology and Forensic Medicine 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Refrigeration Internal medicine Acetylglucosaminidase Enzyme Stability Freezing medicine Humans Transferase Glucosaminidase Molecular Biology chemistry.chemical_classification Kidney gamma-Glutamyltransferase 04 agricultural and veterinary sciences Cell Biology In vitro Enzyme medicine.anatomical_structure Endocrinology chemistry Biochemistry Toxicity |
| Zdroj: | Toxicologic Pathology. 25:264-267 |
| ISSN: | 1533-1601 0192-6233 |
| Popis: | In toxicology studies and clinical trials of erythritol, treated animals and human subjects had higher urine gamma-glutamyl transpeptidase [gamma-glutamyl transferase (gamma-GT)] than untreated controls. It has previously been reported that gamma-GT activity in frozen urine decreases with time; therefore, a study was undertaken to examine the effects of storage temperature, time, and the presence of erythritol on the stability of gamma-GT and N-acetyl glucosaminidase in human urine. In this study, it was found that the rate of decrease of the activity of gamma-GT is much greater at -20 degrees C than at -70 degrees C. Variation in the storage temperature of the frozen urine is particularly deleterious to gamma-GT. The addition of erythritol in a concentration of 5% reduces this decrease. Approximately 15% of N-acetyl glucosaminidase activity is lost in the initial process of freezing the urine. Thereafter, conditions of temperature, time, and the presence or absence of erythritol account for little additional loss of activity. |
| Databáze: | OpenAIRE |
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