Heterologous Overexpression of Human NEFA and Studies on the Two EF-Hand Calcium-Binding Sites

Autor: Stefan Otte, Hartmut Kratzin, Norbert Hilschmann, Shitsu Barnikol-Watanabe, Barnikol Hu, Hilde Götz, Katja A. Kroll, Hans Sternbach, Gregor Hirschfeld
Rok vydání: 1999
Předmět:
Zdroj: Biochemical and Biophysical Research Communications. 260:1-8
ISSN: 0006-291X
DOI: 10.1006/bbrc.1999.0867
Popis: Human NEFA is an EF-hand, leucine zipper protein containing a signal sequence. To confirm the calcium binding capacity of NEFA, recombinant NEFA analogous to the mature protein and mutants with deletions in the EF-hand domain were expressed in Pichia pastoris and secreted into the culture medium at high yield. The calcium binding activity of each purified protein was measured by a modified equilibrium dialysis using the fluorescent Ca2+ indicator FURA-2 and atomic absorption spectroscopy. A stoichiometry of 2 mol Ca2+/mol NEFA was determined. The Ca2+ binding constants were resolved by intrinsic fluorescence spectroscopy. Fluorescence titration exhibited two classes of Ca2+ binding sites with Kd values of 0.08 microM and 0.2 microM. Circular dichroism (CD) spectroscopy showed an increase from 30 to 43% in the amount of alpha-helix in NEFA after addition of calcium ions. Limited proteolytic digestion indicated a Ca2+ dependent conformational change accompanied by an altered accessibility to the enzyme.
Databáze: OpenAIRE