Pattern of HER-2 Gene Amplification and Protein Expression in Benign, Borderline, and Malignant Ovarian Serous and Mucinous Neoplasms
| Autor: | Rabab Mohammed, Tarek M. Elsaba, Rania Makboul, Omar M. Shaaban, Dalia A.H. Elsers, Abeer M.A.B. Thalab |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Pathology medicine.medical_specialty Serous carcinoma Receptor ErbB-2 Receptor expression Chromogenic in situ hybridization Pathology and Forensic Medicine 03 medical and health sciences Young Adult 0302 clinical medicine medicine Carcinoma Biomarkers Tumor Mucinous carcinoma Humans Cystadenocarcinoma Fallopian Tubes In Situ Hybridization Aged Retrospective Studies Aged 80 and over Ovarian Neoplasms business.industry Ovary Gene Amplification Obstetrics and Gynecology Middle Aged medicine.disease Adenocarcinoma Mucinous Immunohistochemistry Cystadenocarcinoma Serous Serous fluid 030104 developmental biology Tissue Array Analysis 030220 oncology & carcinogenesis Adenocarcinoma Female business Precancerous Conditions |
| Zdroj: | International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists. 36(1) |
| ISSN: | 1538-7151 |
| Popis: | Amplification of HER-2 gene and overexpression of HER-2 receptor play a significant role in the progression of a number of malignancies such as breast cancer. Trastuzumab (anti-HER-2 therapeutic agent) has been used successfully in treatment of breast cancer. The aim of this study was to assess the pattern of HER-2 gene amplification and of HER-2 receptor expression in a spectrum of serous and mucinous ovarian tumors to determine whether HER-2 is altered in these neoplasms similar to that occurring in breast cancer. Formalin-fixed paraffin-embedded microarray tissue sections from 212 specimens were stained with HER-2 antibody using immunohistochemistry and with anti-HER-2 DNA probe using chromogenic in situ hybridization. Specimens consisted of 65 benign tumors (50 serous and 15 mucinous), 26 borderline (13 serous and 13 mucinous), 73 malignant tumors (53 serous carcinoma and 20 mucinous carcinoma), 18 metastatic deposits (13 serous and 5 mucinous), in addition to 30 normal tissues (16 ovarian surface and 14 normal fallopian tube). HER-2 protein-positive expression was not detected in the normal or the benign tissues. Borderline neoplasms showed positive staining, but no overexpression. HER-2 overexpression was seen only in 4 carcinoma specimens: 1/53 (1.8%) primary serous carcinomas and 3/20 (15%) primary mucinous carcinomas. HER-2 gene amplification was seen in 4 specimens: 2 primary mucinous carcinomas and 2 malignant deposits of these 2 mucinous carcinomas. In conclusion, alteration of HER-2 was not detected in ovarian serous neoplasms; however, in mucinous carcinoma, HER-2 amplification and overexpression occur. |
| Databáze: | OpenAIRE |
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