Constant infusion and bolus injection of stable-label tracer give reproducible and comparable fasting HGO
| Autor: | D. Halliday, C. E. Robinson, S. Lettis, D. J. A. Eckland, Roman Hovorka, A. Bye, P. Bannister, M. A. Young |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Blood Glucose Male Radioisotope Dilution Technique medicine.medical_specialty Time Factors Hepatic glucose Physiology Endocrinology Diabetes and Metabolism Coefficient of variation Isotope dilution Models Biological Physiology (medical) Internal medicine TRACER medicine Humans Infusions Intravenous Bolus injection Carbon Isotopes Reproducibility Chemistry Reproducibility of Results Deuterium Glucose Endocrinology Liver metabolism Liver Injections Intravenous Constant infusion |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1997.273.1.e192 |
| Popis: | We have investigated the reproducibility of fasting hepatic glucose output (HGO) estimates by use of isotope dilution methodology of stable-label tracers. Six normal subjects were studied on two occasions 1 wk apart. After an overnight fast, the subjects received a bolus injection of 7 mg/kg of [U-13C]glucose and, simultaneously, a primed constant infusion of 0.05 mg.kg-1.min-1 of [6,6(-2)H]glucose. The bolus injection provided one estimate of HGO (HGOBOL), and the constant infusion provided two estimates of HGO, namely, HGO at 2 h (HGOINF2) and HGO at 4 h (HGOINF4), both with the assumption of steady-state conditions. All estimates were similar in value; HGOBOL was highest, followed by HGOINF2 and HGOINF4 [2.30 +/- 0.11 (SE), 2.17 +/- 0.12, and 2.01 +/- 0.13 mg.kg-1.min-1]. The constant infusion gave highly reproducible results. In the case of HGOINF2, the within-subject coefficient of variation (CV) was only 3% compared with 5% of HGOINF4. The reproducibility of HGOBOL was comparable with the within-subject CV of 7%. We conclude that a constant infusion and a bolus injection of stable-label tracer give reproducible and comparable estimates of HGO. |
| Databáze: | OpenAIRE |
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