Molecular characterization and comparison ofClostridium botulinumtype C avian strains

Autor:	Anna Aspán, Hanna Skarin, Anna Lindberg, Gunilla Blomqvist, Viveca Båverud
Rok vydání:	2010
Předmět:	DNA Bacterial Molecular Sequence Data Clostridium botulinum type C Biology medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology SmaI Charadriiformes Bacterial Proteins Food Animals law RNA Ribosomal 16S Pulsed-field gel electrophoresis medicine Animals Botulism Avian botulism Amino Acid Sequence Phylogeny Poultry Diseases Polymerase chain reaction Sweden Base Sequence General Immunology and Microbiology Bird Diseases Intracellular Signaling Peptides and Proteins Genetic Variation Sequence Analysis DNA medicine.disease Electrophoresis Gel Pulsed-Field Random Amplified Polymorphic DNA Technique RAPD Clostridium botulinum Animal Science and Zoology Carrier Proteins Chickens
Zdroj:	Avian Pathology. 39:511-518
ISSN:	1465-3338 0307-9457
DOI:	10.1080/03079457.2010.526923
Popis:	Type C botulinum neurotoxin (BoNT/C)-producing Clostridium botulinum causes animal botulism worldwide and has become a serious problem in poultry flocks and waterfowl in Sweden. The objectives of the present study were to isolate, characterize and subtype C. botulinum type C avian isolates in order to increase the knowledge of the genetic diversity. Isolates from 13 birds were identified by 16S rRNA sequencing and BoNT/C gene detection by real-time polymerase chain reaction (PCR). Conventional PCR was used to distinguish a chimeric BoNTC/D gene, often associated with avian botulism, from the BoNT/C gene. The isolates analysed all contained the gene coding for a chimeric toxin type C/D. Two fingerprinting techniques, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA analysis (RAPD), were optimized and used to investigate the epidemiological relatedness among the strains. The isolates were divided into three different pulsotypes based upon their restriction profiles for SmaI and SalI. The RAPD system proved to be as discriminative as PFGE. This study reveals a small genetic diversity among Swedish type C strains, with a high similarity between strains from broilers and herring gulls.
Databáze:	OpenAIRE
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