Expression and characterization of the integrase of bovine immunodeficiency virus
| Autor: | Amnon Hizi, Orna Avidan |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Immunodeficiency Virus
Bovine LTR Molecular Sequence Data Gene Products pol dUTPase-like segment The lentiviruses BIV Jembrana disease virus and HIV-1 Integrase Homology (biology) Substrate Specificity Strand transfer Virology Reverse transcriptase Animals Humans Amino Acid Sequence Pyrophosphatases Jembrana disease virus Disintegration HIV Long Terminal Repeat chemistry.chemical_classification Base Sequence Integrases biology Terminal Repeat Sequences Bovine immunodeficiency virus biology.organism_classification Molecular biology Enzyme chemistry Lentivirus HIV-1 biology.protein Cattle 3′-end processing Plasmids |
| Zdroj: | Virology. 371:309-321 |
| ISSN: | 0042-6822 |
| Popis: | In the pol gene of bovine immunodeficiency virus (BIV) there is a sequence, located between the reverse-transcriptase and integrase (IN)-encoding sequences, that is not found in HIV-1 pol gene and encodes a 74-residue polypeptide with homology to dUTPases. We have expressed two BIV IN versions that differ in their amino termini. The longer version, containing the 74-residue sequence, did not show any detectable 3′-end processing and strand transfer IN activities and performed only the IN-associated disintegration. Consequently, the shorter version, lacking the dUTPase-related residues, performed all three activities and is most likely similar to the viral enzyme. A comparison between BIV IN and the well-studied HIV-1 IN, with substrates that mimic the U5 LTR sequences of BIV, HIV-1 and another bovine lentivirus, Jembrana disease virus, revealed that the extra 3′-end sequence beyond the conserved “CA” is probably less important for IN activities than the sequence upstream to the “CA". |
| Databáze: | OpenAIRE |
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