Effects of omega-3 polyunsaturated fatty acids on steroidogenesis and cellular development in PCOS rats
| Autor: | Yanzhou Yang, Cheng Zhang, Ye Tian, Chengtao Wang, Xusong Hu, Xuechun Weng, Kaili Xu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Steroid biosynthesis Rats Sprague-Dawley 03 medical and health sciences Phosphatidylinositol 3-Kinases Internal medicine Fatty Acids Omega-3 medicine Animals Humans PI3K/AKT/mTOR pathway Progesterone Cell Proliferation chemistry.chemical_classification Gene knockdown 030109 nutrition & dietetics Granulosa Cells Estradiol Cell growth Hyperandrogenism General Medicine Metabolism medicine.disease Polycystic ovary Rats 030104 developmental biology Endocrinology chemistry Cytochrome P450 Family 51 Female Food Science Polyunsaturated fatty acid Polycystic Ovary Syndrome |
| Zdroj: | Foodfunction. 10(5) |
| ISSN: | 2042-650X |
| Popis: | Polycystic ovary syndrome (PCOS) is a common endocrine disorder, which is characterized by hyperandrogenism. Polyunsaturated fatty acids (PUFAs) are necessary for the body's metabolism, growth and development. Despite the well-known benefits of omega-3 polyunsaturated fatty acid supplementation on the modulation of PCOS ovarian function, relatively little is known about the precise regulation mechanism. The objective of this study was to determine the cellular and molecular mechanisms by which omega-3 regulates CYP51 expression and steroid biosynthesis during follicle growth in PCOS. The results indicated that the CYP51 expression was up-regulated in granulosa cells by omega-3. Moreover, the knockdown of CYP51 blocked omega-3 induced estradiol (E2) and progesterone (P4) synthesis as well as cellular viability and proliferation. These changes were accompanied by the up-regulation of the p-Akt level. Furthermore, the PI3K/Akt pathway was required for the regulation of CYP51 expression, steroidogenesis and cell development by omega-3 in PCOS granulosa cells. Our data demonstrate that omega-3 potentiates the cellular development and steroid biosynthesis via CYP51 up-regulation in PCOS, which are mediated through the activation of the PI3K/Akt pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|