Do trifluralin and tebuthiuron impair isolated rat liver mitochondria?
| Autor: | Mariana Furio Franco-Bernanrdes, Daniel Junqueira Dorta, Murilo Pazin, Bárbara de Oliveira, Lilian Cristina Pereira |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Center for Evaluation of Environmental Impact on Human Health (TOXICAM), Universidade de Brasília (UnB), Unidade Águas Claras |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Health Toxicology and Mutagenesis Mitochondria Liver Mitochondrion medicine.disease_cause 01 natural sciences RATOS 03 medical and health sciences chemistry.chemical_compound Tebuthiuron medicine Animals Inner mitochondrial membrane Emerging contaminants Methylurea Compounds Herbicides Trifluralin General Medicine Glutathione Mitochondria Rats 010602 entomology 030104 developmental biology chemistry Biochemistry Toxicity NAD+ kinase Agronomy and Crop Science Oxidative stress |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2020-12-12T01:48:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01 Universidade de São Paulo Emerging contaminants, such as the herbicides trifluralin and tebuthiuron, comprise a class of compounds for which toxicological data are lacking, especially data regarding their harmful effects and biomarkers of exposure. Their potential damage to the environment and non-target organisms makes understanding their toxic mechanisms an urgent matter. Mitochondria, which exert an energy production function, play a vital role in maintaining many cellular activities and therefore are reliable predictors of substance toxicity. This study evaluates whether the herbicides trifluralin and tebuthiuron (at concentrations ranging from 1 to 100 μM) affect isolated rat liver mitochondria. The herbicides were analyzed according to their ability to interact with the mitochondrial membrane and induce swelling, lipoperoxidation, ROS formation, and NAD(P)H oxidation; dissipate the membrane potential; dysregulate calcium homeostasis; and alter ATP and GSH/GSSG levels. Tebuthiuron does not disrupt the mitochondrial biochemistry at any of the tested concentrations. In contrast, trifluralin can disturb the mitochondrial respiration, especially at the highest concentration, but it cannot induce oxidative stress. These results suggest that the aforementioned effects can occur as toxic mechanisms of trifluralin in non-target organisms, as well. Faculty of Pharmaceutical Sciences of Ribeirão Preto Department of Clinical Toxicological and Bromatological Analyzes University of São Paulo Department of Bioprocesses and Biotechnology Faculty of Agronomic Sciences of Botucatu São Paulo State University Center for Evaluation of Environmental Impact on Human Health (TOXICAM) Faculdade de Ceilândia (FCE) Universidade de Brasília (UnB), Distrito Federal Centro Universitário ICESP Unidade Águas Claras, Distrito Federal Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Departamento de Química Universidade de São Paulo Instituto Nacional de Tecnologias Alternativas de Detecção Avaliação Toxicológica e Remoção de Micropututantes e Radioativos (INCT-DATREM) Unesp Instituto de Química, Caixa Postal 355, CEP: 14800-900 Department of Bioprocesses and Biotechnology Faculty of Agronomic Sciences of Botucatu São Paulo State University Instituto Nacional de Tecnologias Alternativas de Detecção Avaliação Toxicológica e Remoção de Micropututantes e Radioativos (INCT-DATREM) Unesp Instituto de Química, Caixa Postal 355, CEP: 14800-900 |
| Databáze: | OpenAIRE |
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