Effect of exogenous administration of Candida albicans autoregulatory alcohols in a murine model of hematogenously disseminated candidiasis
| Autor: | Margarida Isabel Barros Coelho Martins, Jose L. Lopez-Ribot, Mariana Henriques, Rosário Oliveira, Anna L. Lazzell |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2011 |
| Předmět: |
Antifungal Agents
Autoregulatory alcohols Biology Kidney Applied Microbiology and Biotechnology Microbiology Mice 03 medical and health sciences Candida albicans Animals 030304 developmental biology 0303 health sciences Science & Technology 030306 microbiology Candidiasis General Medicine biology.organism_classification Disseminated Candidiasis Survival Analysis Growth Inhibitors 3. Good health Disease Models Animal Murine model Alcohols |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0233-111X |
| DOI: | 10.1002/jobm.201100158 |
| Popis: | Candida albicans supernatants contain a mixture of autoregulatory alcohols. In vitro, when added individually or in combination, these alcohols inhibit the yeast to filamentous form conversion. Here we evaluate the in vivo effect of the exogenous administration of a Cocktail solution simulating the composition of alcohols present in a C. albicans culture supernatant(1 ml; 94 μmol l–1 isoamyl alcohol, 70 μmol l–1 2-phenylethanol, 3.2 n mol l–1 E-nerolidol, and 18 n mol l–1 E,E-farnesol) using the well established murine model of hematogenously disseminated candidiasis. Mice injected intraperitoneally with the Cocktail solution demonstrated increased survival and decreased organ fungal burden compared to control mice. Histological observations suggest that the Cocktail, to some extent, has an inhibitory effect on cell filamentation within the kidney. These findings suggest that the exogenous administration of C. albicans autoregulatory alcohols displays a protective effect during disseminated candidiasis. M. M. was financially supported by a fellowship from Fundacao para a Ciencia e Tecnologia, Portugal (contracts SFRH/BD/28222/2006 and SFRH/BPD/73663/2010), co-financed by the Programa Operacional Potencial Humano (POPH)/Fundo Social Europeu (FSE). Work in the laboratory of J.L.L.-R. is supported by grant number R21AI080930 from the National Institute of Allergy and Infectious Diseases. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIAID or the NIH. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text