Nitrogen deprivation of Anabaena sp. strain PCC 7120 elicits rapid activation of a gene cluster that is essential for uptake and utilization of nitrate
| Autor: | Y Cai, C P Wolk |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Transcriptional Activation
Transposable element Nitrite Reductases Nitrogen Recombinant Fusion Proteins Molecular Sequence Data Restriction Mapping Nitrate reductase Nitrate Reductase Microbiology Genes Reporter Nitrate Reductases Transcription (biology) Luciferase Amino Acid Sequence Cloning Molecular Luciferases Molecular Biology Gene Regulation of gene expression Nitrates Base Sequence Sequence Homology Amino Acid biology Anabaena Ferredoxin-Nitrite Reductase Gene Expression Regulation Bacterial Sequence Analysis DNA biology.organism_classification Nitrite reductase Biochemistry Genes Bacterial Multigene Family DNA Transposable Elements Research Article |
| Zdroj: | Journal of Bacteriology. 179:258-266 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.179.1.258-266.1997 |
| Popis: | A transposon bearing luxAB, encoding luciferase, as a reporter of transcription was used to identify genes that are activated rapidly upon deprivation of Anabaena sp. strain PCC 7120 of fixed nitrogen. The three transposon-marked loci that were identified as responding most rapidly and strongly are closely linked and situated within nirA and nrtC and between nrtD and narB, genes whose products are responsible for uptake and reduction of NO2- and NO3-. A strain bearing a transcriptional fusion of narB to luxAB was constructed. Luminescence catalyzed by LuxAB was used to report on the expression of the interrupted genes. Whether these genes are regulated only coordinately is discussed. |
| Databáze: | OpenAIRE |
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