Advances in Microfluidics and Lab-on-a-Chip Technologies
| Autor: | Huizhong Li, J. Nelson, Valentin Romanov, Harikrishnan Jayamohan, Raheel Samuel, Bruce K. Gale, Jiyoung Son |
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| Rok vydání: | 2016 |
| Předmět: |
Biodefense
Lab-on-a-chip 010401 analytical chemistry Microfluidics Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Molecular diagnostics 01 natural sciences Protein detection 0104 chemical sciences law.invention Polymerase chain reaction Isothermal amplification Cell sorting Cytogenetics law High-resolution melting analysis Next-generation sequencing Enhanced sensitivity Molecular diagnostic techniques 0210 nano-technology |
| Popis: | Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense. The emergence of microfluidics and nanotechnologies has enabled both new capabilities and instrument sizes practical for point-of-care. It has also introduced new functionality, enhanced sensitivity, and reduced the time and cost involved in conventional molecular diagnostic techniques. This chapter reviews the application of microfluidics for molecular diagnostics methods such as nucleic acid amplification, next-generation sequencing, high-resolution melting analysis, cytogenetics, protein detection and analysis, and cell sorting. We also review microfluidic sample preparation platforms applied to molecular diagnostics and targeted to sample-in, answer-out capabilities. |
| Databáze: | OpenAIRE |
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