Detection of a canine parvovirus type 2c with a non-coding mutation and its implications for molecular characterisation
| Autor: | Michele Losurdo, Nicola Decaro, Vito Martella, Antonio Parisi, Gianpiero Ventrella, Gabriella Elia, Francesca Amorisco, Canio Buonavoglia, Costantina Desario |
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| Rok vydání: | 2012 |
| Předmět: |
Silent mutation
Gene Expression Regulation Viral Parvovirus Canine Sequence analysis animal diseases viruses Mutant Biology Diagnostic tools medicine.disease_cause Real-Time Polymerase Chain Reaction law.invention Parvoviridae Infections Dogs law medicine Animals Dog Diseases Polymerase chain reaction Phylogeny Genetics Mutation General Veterinary Strain (chemistry) Canine parvovirus biology.organism_classification Virology Animal Science and Zoology |
| Zdroj: | Veterinary journal (London, England : 1997). 196(3) |
| ISSN: | 1532-2971 |
| Popis: | An epidemiological survey for canine parvovirus (CPV) was conducted by collecting 615 faecal samples from dogs with diarrhoea in different European countries. Molecular methods showed that CPV-2a was predominant in most countries, followed by CPV-2c and CPV-2b, whereas 30 strains were not characterised. By sequence analysis of the full-length VP2 gene, 20 of these viruses were characterised as CPV-2c mutants having the synonymous mutation A4061G in the probe-binding region that prevented correct strain characterisation. A real-time polymerase chain reaction (PCR) assay using a minor groove binder probe was able to recognise both mutant and classical CPV-2c strains. These results indicate that the emergence of CPVs with mutations affecting the oligonucleotide-binding region needs a continuous update of molecular diagnostic tools in order to detect efficiently those emerging strains. |
| Databáze: | OpenAIRE |
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