Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2
| Autor: | L. A. Oleinik, V. A. Svyatchenko, Pavel Madonov, A. A. Kotlyarova, German Baikalov, S. S. Legostaev, N. A. Kikhtenko |
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| Rok vydání: | 2021 |
| Předmět: |
Serial dilution
viruses Cell Gene Expression Virus Replication medicine.disease_cause Antiviral Agents Article General Biochemistry Genetics and Molecular Biology Virus law.invention law Chlorocebus aethiops Escherichia coli medicine Animals Humans Cloning Molecular Vero Cells Coronavirus Strain (chemistry) SARS-CoV-2 Chemistry COVID-19 General Medicine Viral Load Virology Recombinant Proteins In vitro COVID-19 Drug Treatment medicine.anatomical_structure Cell culture Recombinant DNA Interferons recombinant human interferon λ-1 specific antiviral activity |
| Zdroj: | Bulletin of Experimental Biology and Medicine |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-021-05330-0 |
| Popis: | The antiviral activity of recombinant human IFN-lambda type 1 (IFNλ-1) against culture strain of SARS-CoV-2 virus was determined by infecting a highly sensitive VeroE6 coronavirus cell culture after preincubation test (the cell monolayer was incubated with 4-fold dilutions of IFNλ-1 in a concentration range of 0.16-42,500 ng/ml in a culture medium for 12 h at 37°C) and without preincubation (simultaneous addition of different concentrations of IFNλ-1 and SARS-CoV-2 infection in a dose of 102 TCID50). The created recombinant human IFNλ-1 demonstrated obvious antiviral activity against SARS-CoV-2 virus in vitro. In the tests with and without preincubation, IFNλ-1 exhibited significant activity, although somewhat lower in variant with simultaneous addition of IFNλ-1 and virus to the cell culture. It should be noted that the antiviral effect of IFNλ-1 was observed in a wide range of concentrations. |
| Databáze: | OpenAIRE |
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