EFFECT OF A PROTEIN PHOSPHATASE INHIBITOR, OKADAIC ACID, ON THROMBOMODULIN EXPRESSION IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
| Autor: | Shuichi Horie, Hajime Maeda, Mitsuyuki Kohno, Hidemi Ishii, Tsuguhiko Nakai, Koji Oida |
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| Rok vydání: | 1997 |
| Předmět: |
Umbilical Veins
Thrombomodulin Phosphatase Cell Biology Umbilical vein chemistry.chemical_compound Okadaic Acid Phosphoprotein Phosphatases medicine Humans RNA Messenger Cycloheximide Enzyme Inhibitors Cells Cultured chemistry.chemical_classification Hematology Okadaic acid Blotting Northern Molecular biology Enzyme medicine.anatomical_structure chemistry Cell culture Endothelium Vascular Protein C medicine.drug |
| Zdroj: | Thrombosis Research. 85:169-176 |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/s0049-3848(97)00001-7 |
| Popis: | We examined the effects of okadaic acid, a potent specific inhibitor of protein phosphatases 1 and 2A, on the expression of thrombomodulin (TM), a cell surface anti-thrombotic glycoprotein, on cultured human umbilical endothelial cells. Okadaic acid (2.5–10 nM) significantly increased TM antigen levels in parallel with its cofactor activity for thrombin-dependent protein C activation. Incubation of cells with 10 nM okadaic acid for 18 h induced an approximately 240% up-regulation of TM antigen levels that was accompanied by an increase in TM mRNA levels. Co-incubation of cells with okadaic acid and dibutyryl cyclic AMP further increased TM antigen levels. Furthermore, the effect of cAMP on TM expression was augmentated by the pre-treatment of cells with 10 nM okadaic acid for 18 h. These results provide evidence for the involvement of protein phosphatase in the cellular regulatory mechanisms for TM expression, which is distinct from that by cAMP. Copyright © 1997 Elsevier Science Ltd |
| Databáze: | OpenAIRE |
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