Comparison of the binding of the human papillomavirus type 16 and cottontail rabbit papillomavirus E7 proteins to the retinoblastoma gene product
| Autor: | G A Vuocolo, Kathleen M. Haskell, Mona Ivey-Hoyle, Raymond E. Jones, Deborah Defeo-Jones |
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| Rok vydání: | 1993 |
| Předmět: |
Papillomavirus E7 Proteins
Molecular Sequence Data Cottontail rabbit papillomavirus Retinoblastoma Protein Virus Gene product Virology Animals Humans Amino Acid Sequence Human papillomavirus Binding site Papillomaviridae Cellular Transformation Sequence Homology Amino Acid biology fungi Retinoblastoma protein virus diseases Oncogene Proteins Viral Molecular biology female genital diseases and pregnancy complications biology.protein Retinoblastoma gene Rabbits Protein Binding |
| Zdroj: | Journal of General Virology. 74:115-119 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Binding of the human papillomavirus type 16 (HPV-16) E7 oncoprotein to the retinoblastoma protein (pRb) is thought to be involved in the cellular transformation mediated by HPV-16. Here we show that the E7 protein of the cottontail rabbit papillomavirus (CRPV) binds to the same C-terminal portion of human pRb as HPV-16 E7, and that both the CRPV and HPV-16 E7 proteins bind specifically through similar domains to rabbit pRb. Furthermore, a single amino acid substitution which reduces the binding of HPV-16 E7 to human pRb also abolishes binding of CRPV E7 to both human and rabbit pRb. The biochemical similarities observed between the HPV-16 and CRPV E7 proteins suggest that they are functionally conserved. These results further validate the use of CRPV as an animal model for the study of HPV-mediated disease. |
| Databáze: | OpenAIRE |
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