Further studies of the physical and metabolic properties of foot-and-mouth disease virus temperature-sensitive mutants
| Autor: | J Y Richmond, J Polatnick |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Immunology
Mutant Viral Plaque Assay In Vitro Techniques medicine.disease_cause Microbiology Virus chemistry.chemical_compound Aphthovirus medicine Virus quantification Mutation biology DEAE-Dextran Temperature RNA Hydrogen-Ion Concentration biology.organism_classification Molecular biology Uridine Infectious Diseases chemistry RNA Viral Parasitology Foot-and-mouth disease virus Research Article |
| Zdroj: | Infection and Immunity. 13:1392-1396 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.13.5.1392-1396.1976 |
| Popis: | Three temperature-sensitive (ts) mutants of foot-and-mouth disease virus were classified as ribonucleic acid negative and as belonging to the same complementation group when measured by virus yields and [3H] uridine incorporation in paired, mixed infections at the nonpermissive temperature (38.5C). Mutant ts-22, the only mutant able to produce plaques at 38.5 C, was more sensitive to acid than were the parental wild-type or other mutant viruses. Diethylaminoethyl-dextran did not enhance the plaque-forming ability of the mutant viruses at 38.5C. All of the viruses inhibited host cell protein syntehsis at both permissive (33C) and nonpermissive (38.5C) temperatures. |
| Databáze: | OpenAIRE |
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