IGF-I, IGF-II, and IGF-receptor-1 transcript and IGF-II protein expression in myostatin knockout mice tissues
| Autor: | Lori Batelli, Scott A. Gahr, Ann F. Hubbs, John Killefer, Hakan Kocamiş |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Physiology medicine.medical_treatment Myostatin Biology Insulin-Like Growth Factor Receptor Kidney Receptor IGF Type 1 Muscle hypertrophy Mice Cellular and Molecular Neuroscience Insulin-like growth factor Insulin-Like Growth Factor II Transforming Growth Factor beta Physiology (medical) Internal medicine medicine Animals RNA Messenger Insulin-Like Growth Factor I Muscle Skeletal Mice Knockout Soleus muscle Reverse Transcriptase Polymerase Chain Reaction Myocardium Brain Skeletal muscle musculoskeletal system Immunohistochemistry Actins Mice Inbred C57BL medicine.anatomical_structure Endocrinology Liver Organ Specificity Insulin-like growth factor 2 Knockout mouse biology.protein Neurology (clinical) |
| Zdroj: | Muscle & Nerve. 26:55-63 |
| ISSN: | 1097-4598 0148-639X |
| DOI: | 10.1002/mus.10160 |
| Popis: | Semiquantitative reverse transcriptase–polymerase chain reaction (RT-PCR) and immunohistochemistry were performed to demonstrate whether a correlation exists between insulin-like growth factors (IGFs)—positive regulators of growth—and myostatin, a negative regulator of muscle growth. IGF-I, -II, and IGF–receptor-1 (IGF-R1) mRNA and IGF-II protein expressions were determined in control and myostatin knockout mice tissues. IGF-I gene expressions were similar between control and knockout mice tissues, whereas IGF-II mRNA levels were significantly higher in myostatin knockout mice kidney and soleus muscles than those of control mice (P < .01). IGF-R1 mRNA levels from control mice heart (P < .05) and kidney (P < .01) were significantly higher than in myostatin knockout mice, whereas levels were lower in pectoralis muscle of control mice than knockout mice (P < .01). The strongly IGF-II–positive cells in soleus muscle were more common in myostatin knockout mice and were seen in a few foci in control mice. IGF–II immunoreactivity in both control and myostatin knockout mice kidneys was localized to the epithelium of renal tubules and collecting ducts. Reciprocal changes in the expression of myostatin and IGF-II and IGF-R1 may underlie normal growth of skeletal muscle and other organs in mammals, and the changes in these tissues associated with disease. © 2002 Wiley Periodicals, Inc. Muscle Nerve 26: 55–63, 2002 |
| Databáze: | OpenAIRE |
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