Measuring intraocular antibodies in eyes treated with anti-vascular endothelial growth factor
| Autor: | Manpartap Singh Bal, Tom Gonder, Parsa Mehraban Far, Todd Urton, Steven Bae, Robert L. Campbell, Vasudha Gupta, Jacob Rullo, Mark D. Bona, Wilma M. Hopman, Amro Al Hazimi, Isabella Irrcher, Sanjay Sharma |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
Immunoglobulin A medicine.medical_specialty genetic structures medicine.medical_treatment Angiogenesis Inhibitors Macular Edema Immunoglobulin G Aqueous Humor chemistry.chemical_compound Ophthalmology medicine Humans Macular edema biology business.industry Aqueous humour Retinal General Medicine Diabetic retinopathy Cataract surgery medicine.disease eye diseases Bevacizumab Cross-Sectional Studies chemistry Immunoglobulin M Intravitreal Injections biology.protein sense organs business |
| Zdroj: | Canadian Journal of Ophthalmology. 55:263-271 |
| ISSN: | 0008-4182 |
| Popis: | Objective To characterize the total intraocular aqueous humour antibody profiles in cases receiving anti-vascular endothelial growth factor (anti-VEGF) for retinal vascular disease compared with controls without retinal pathology. Design Cross-sectional. Participants 93 aqueous humour samples: 22 eyes undergoing cataract surgery (controls) and 71 eyes receiving intravitreal injections (IVI) (cases) for macular edema or neovascularization. Methods Antibody isotyping of aqueous humour was performed using Milliplex MAP Human Isotyping Multiplex Assay. Cases and controls were compared for several outcome measures. Results The primary outcome measure was total mean antibody isotype concentration quantified in the aqueous humour. Secondary outcomes included comparing aqueous humour concentrations with visual acuity, number of IVI received, type of anti-VEGF agent injected, and persistence intra-/subretinal fluid post injection. Mean immunoglobulin M (IgM) concentrations in cases were 19-fold higher compared with controls. Aqueous immunoglobulin G (IgG)1,2,3,4 and immunoglobulin A (IgA) were 2–4-fold higher in cases compared with controls. Disease-specific trends were observed, with diabetic retinopathy (DR) eyes containing the highest amounts of aqueous antibodies. Total number of injections correlated with higher titres of IgG1 (p Conclusions Intraocular antibodies are present in the aqueous humour at significantly higher concentrations in eyes receiving IVIs for retinal vascular diseases compared with controls. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect