Selection of prosomes and prosomal RNA by immobilized viral RNAs
| Autor: | Kurt Köhler, Andrea Horsch, Hans-Peter Schmid, Marlies Ellwart-Tschürz |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
viruses
Biophysics Prosome RNA-dependent RNA polymerase Prosomal RNA Biology Viral mRNA Affinity chromatography Cell Fractionation Biochemistry Virus Chromatography Affinity Nucleic acid thermodynamics Structural Biology Genetics Tobacco mosaic virus Humans RNA Messenger Molecular Biology Ribonucleoprotein Messenger RNA Adenoviruses Human RNA Nucleic Acid Hybridization Tobamovirus Cell Biology biology.organism_classification Molecular biology Cell biology Globins Tobacco Mosaic Virus Ribonucleoproteins RNA Viral Electrophoresis Polyacrylamide Gel Hybrid selection HeLa Cells |
| Zdroj: | FEBS letters. 269(2) |
| ISSN: | 0014-5793 |
| Popis: | Viral messengers were used to select and purify prosomes and prosomal RNA from subribosomal fractions of HeLa cells and mouse erythroblasts. Adenovirus mRNA immobilized on oligo(dT)-cellulose and tobacco mosaic virus RNA (TMV) sedimenting in sucrose gradients associated strongly with prosomes at high salt conditions forming intermolecular RNA-RNA hybrids between prosomal RNA and viral RNA. Hybrid selection of small cytoplasmic RNAs with immobilized TMV-RNA revealed a RNA species migrating at the same position as prosomal RNA. The possible existence of a box-like sequence involved in hybridization will be discussed. |
| Databáze: | OpenAIRE |
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