18S rRNA processing requires base pairings of snR30 H/ACA snoRNA to eukaryote-specific 18S sequences

Autor: Tamás Kiss, Yves Henry, Vera Atzorn, Eléonore Fayet-Lebaron
Přispěvatelé: Laboratoire de biologie moléculaire eucaryote (LBME), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
RNA
Untranslated
non-coding RNA
MESH: DNA Breaks
Double-Stranded
MESH: Base Sequence
Conserved sequence
MESH: RNA
Small Nuclear
MESH: RNA
Untranslated
MESH: Saccharomyces cerevisiae Proteins
MESH: Structure-Activity Relationship
MESH: Centrifugation
Density Gradient
RNA
Small Nuclear
MESH: Gene Conversion
Small nucleolar RNA
RNA structure
Base Pairing
rRNA processing
Genetics
MESH: DNA Repair
0303 health sciences
General Neuroscience
030302 biochemistry & molecular biology
small nucleolar RNA
Fungal genetics
Non-coding RNA
MESH: Saccharomyces cerevisiae
MESH: Chromosomes
Fungal
MESH: Nucleic Acid Conformation
MESH: Recombination
Genetic
MESH: Cell Nucleus
MESH: Mutation
Molecular Sequence Data
MESH: Base Pairing
Saccharomyces cerevisiae
box H/ACA snoRNA
Biology
MESH: Genes
Mating Type
Fungal
Article
General Biochemistry
Genetics and Molecular Biology
Dyskerin
MESH: Rad52 DNA Repair and Recombination Protein
Structure-Activity Relationship
03 medical and health sciences
MESH: Nuclear Envelope
Centrifugation
Density Gradient
RNA
Ribosomal
18S
MESH: Protein Binding
MESH: Blotting
Northern
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
RRNA processing
Molecular Biology
MESH: Antigens
Nuclear
030304 developmental biology
MESH: Molecular Sequence Data
Base Sequence
General Immunology and Microbiology
MESH: RNA
Fungal
RNA
RNA
Fungal
Ribosomal RNA
Blotting
Northern
MESH: RNA
Ribosomal
18S
MESH: Mutagenesis
Insertional
MESH: Operator Regions
Genetic
Nucleic Acid Conformation
MESH: Telomere
MESH: DNA-Binding Proteins
Zdroj: EMBO Journal
EMBO Journal, EMBO Press, 2009, 28 (9), pp.1260-70. ⟨10.1038/emboj.2009.79⟩
The EMBO Journal
ISSN: 0261-4189
1460-2075
DOI: 10.1038/emboj.2009.79⟩
Popis: International audience; The H/ACA RNAs represent an abundant, evolutionarily conserved and functionally diverse class of non-coding RNAs. Many H/ACA RNAs direct pseudouridylation of rRNAs and snRNAs, while members of the rapidly growing group of 'orphan' H/ACA RNAs participate in pre-rRNA processing, telomere synthesis and probably, in other nuclear processes. The yeast snR30 'orphan' H/ACA snoRNA has long been known to function in the nucleolytic processing of 18S rRNA, but its molecular role remained unknown. Here, we provide biochemical and genetic evidence demonstrating that during pre-rRNA processing, two evolutionarily conserved sequence elements in the 3'-hairpin of snR30 base-pair with short pre-rRNA sequences located in the eukaryote-specific internal region of 18S rRNA. The newly discovered snR30-18S base-pairing interactions are essential for 18S rRNA production and they constitute a complex snoRNA target RNA transient structure that is novel to H/ACA RNAs. We also demonstrate that besides the 18S recognition motifs, the distal part of the 3'-hairpin of snR30 contains an additional snoRNA element that is essential for 18S rRNA processing and that functions most likely as a snoRNP protein-binding site.
Databáze: OpenAIRE
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