The effect of continuous ELF-MFs on the level of 5-HIAA in the raphe nucleus of the rat
| Autor: | Leila Shiri, Daryoush Shahbazi-Gahrouei, Hojjatollah Alaei, Naser Naghdi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Nervous system Serotonin medicine.medical_specialty Microdialysis microdialysis Health Toxicology and Mutagenesis Chloral hydrate Serotonergic Radiation Biology High-performance liquid chromatography 03 medical and health sciences 0302 clinical medicine ELF-MF Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Rats Wistar Chromatography High Pressure Liquid Radiation 5-Hydroxyindoleacetic acid Chemistry raphe nuclei Hydroxyindoleacetic Acid Reference Standards respiratory system Magnetic Fields 030104 developmental biology Endocrinology medicine.anatomical_structure Anesthesia Metabolome HPLC 5-hydroxyindoleacetic acid Raphe nuclei 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Radiation Research |
| ISSN: | 1349-9157 0449-3060 |
| DOI: | 10.1093/jrr/rrv093 |
| Popis: | The aim of this study was to investigate the effect of continuous extremely low frequency magnetic fields (ELF-MFs) with a frequency of 10 Hz and an intensity of 690–720 μT on the level of 5-hydroxyindolacetic acid (5-HIAA) in adult male Wistar rats. A total of 24 adult Wistar male rats were used, and after exposure with an ELF-MF for 15 successive days, all rats in each test were anesthetized with chloral hydrate. Then, they were placed in a stereotaxic frame for surgery and a microdialysis process. Dialysate samples were analyzed to measure the amount of 5-HIAA by high performance liquid chromatography (HPLC) using electrochemical detection. Results showed that ELF-MF exposure for 15 days, 1 h daily, was not effective in altering the level of 5-HIAA. However, ELF-MF exposure for 15 days, 3 h daily, decreased the level of the 5-HIAA in the raphe nucleus. It can be concluded that ELF-MFs affect the serotonergic system and may be used to treat nervous system diseases. This study is an initial step towards helping cure depression using ELF-MFs. |
| Databáze: | OpenAIRE |
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