Effect of Glut‐1 and HIF‐1α double knockout by CRISPR/CAS9 on radiosensitivity in laryngeal carcinoma via the PI3K/Akt/mTOR pathway
| Autor: | Yang‐Yang Bao, Jiang‐Tao Zhong, Li‐Fang Shen, Li‐Bo Dai, Shui‐Hong Zhou, Jun Fan, Hong‐Tian Yao, Zhong‐Jie Lu |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Glucose Transporter Type 1
TOR Serine-Threonine Kinases Carcinoma Cell Biology Hypoxia-Inducible Factor 1 alpha Subunit Radiation Tolerance Phosphatidylinositol 3-Kinases Glucose Cell Line Tumor Animals Humans Molecular Medicine CRISPR-Cas Systems Hypoxia Wortmannin Laryngeal Neoplasms Proto-Oncogene Proteins c-akt |
| Zdroj: | Journal of Cellular and Molecular Medicine. 26:2881-2894 |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/jcmm.17303 |
| Popis: | Hypoxic resistance is the main obstacle to radiotherapy for laryngeal carcinoma. Our previous study indicated that hypoxia-inducible factor 1α (HIF-1α) and glucose transporter 1 (Glut-1) double knockout reduced tumour biological behaviour in laryngeal carcinoma cells. However, their radioresistance mechanism remains unclear. In this study, cell viability was determined by CCK8 assay. Glucose uptake capability was evaluated by measurement of |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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