Molecular and Functional Characterization of Mouse S5D-SRCRB: A New Group B Member of the Scavenger Receptor Cysteine-Rich Superfamily
| Autor: | Citlali Vázquez-Echeverría, Dorte Rosenbek Fink, Uffe Holmskov, Francisco Lozano, Carles Serra-Pagès, Cristina Pujades, José Yélamos, Vanesa G. Martínez, Angeles García-Pardo, Paula Espinal-Marin, Cristina Miró-Julià, Olga Padilla, Jordi Vila, Sandra Roselló, Cristina Escoda-Ferran |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Molecular Sequence Data Immunology law.invention Rats Sprague-Dawley Mice Exon law Laminin Complementary DNA Animals Homeostasis Humans Immunology and Allergy Amino Acid Sequence Cysteine Scavenger receptor Receptor Gene map biology Epithelial Cells Scavenger Receptors Class B Molecular biology Immunity Innate Transmembrane protein Protein Structure Tertiary Rats Cell biology Mice Inbred C57BL HEK293 Cells Gene Expression Regulation Multigene Family Receptors Pattern Recognition Recombinant DNA biology.protein Protein Binding |
| Zdroj: | Miró-Julià, C, Roselló, S, Martínez, V G, Fink, D R, Escoda-Ferran, C, Padilla, O, Vázquez-Echeverría, C, Espinal-Marin, P, Pujades, C, García-Pardo, A, Vila, J, Serra-Pagès, C, Holmskov, U, Yélamos, J & Lozano, F 2011, ' Molecular and Functional Characterization of Mouse S5D-SRCRB: A New Group B Member of the Scavenger Receptor Cysteine-Rich Superfamily ', Journal of Immunology, vol. 186, no. 4, pp. 2344-2354 . https://doi.org/10.4049/jimmunol.1000840 |
| Popis: | The scavenger receptor cysteine-rich superfamily (SRCR-SF) members are transmembrane and/or secreted receptors exhibiting one or several repeats of a cysteine-rich protein module of ∼100 aa, named scavenger receptor cysteine-rich (SRCR). Two types of SRCR domains (A or B) have been reported, which differ in the number of coding exons and intradomain cysteines. Although no unifying function has been reported for SRCR-SF members, recognition of pathogen-associated molecular patterns (PAMPs) was recently shown for some of them. In this article, we report the structural and functional characterization of mouse S5D-SRCRB, a new group B member of the SRCR-SF. The s5d-srcrb gene maps at mouse chromosome 7 and encompasses 14 exons extending over 15 kb. The longest cDNA sequence found is 4286 bp in length and encodes a mature protein of 1371 aa, with a predicted Mr of 144.6 kDa. Using an episomal mammalian-expression system, a glycosylated soluble recombinant form >200 kDa was obtained and used as immunogen for the generation of specific rat mAbs. Subsequent immunohistochemical and real-time PCR analysis showed significant S5D-SRCRB expression in murine genitourinary and digestive tracts. S5D-SRCRB was shown to bind endogenous extracellular matrix proteins (laminin and galectin-1), as well as PAMPs present on Gram-positive and Gram-negative bacteria and fungi. PAMP binding by S5D-SRCRB induced microbial aggregation and subsequent inhibition of PAMP-induced cytokine release. These abilities suggest that S5D-SRCRB might play a role in the innate defense and homeostasis of certain specialized epithelial surfaces. |
| Databáze: | OpenAIRE |
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