Rhinovirus infection increases 5-lipoxygenase and cyclooxygenase-2 in bronchial biopsy specimens from nonatopic subjects
| Autor: | Michelle L. Seymour, Phillip G. Bardin, David J. Fraenkel, John F. Penrose, Sebastian L. Johnston, Natalie Gilby, Anthony P. Sampson, Stephen T. Holgate, G. Sanderson |
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| Rok vydání: | 2001 |
| Předmět: |
Leukotrienes
Biopsy Common Cold Bronchi Biology medicine.disease_cause medicine Immunology and Allergy Bronchial Biopsy Humans Cysteine Arachidonate 5-Lipoxygenase medicine.diagnostic_test Respiratory disease Membrane Proteins Common cold respiratory system medicine.disease respiratory tract diseases Isoenzymes Infectious Diseases Bronchoalveolar lavage Bronchial hyperresponsiveness Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Immunology biology.protein Cyclooxygenase Rhinovirus |
| Zdroj: | The Journal of infectious diseases. 185(4) |
| ISSN: | 0022-1899 |
| Popis: | Rhinovirus infections cause wheeze, cough, and bronchial hyperresponsiveness. To investigate the involvement of cysteinyl-leukotrienes and prostanoids in these symptoms, bronchial biopsy specimens from 9 normal subjects (nonatopic and with no history of chronic lung disease) were immunostained for 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathway enzymes 2 weeks before and 4 days after experimental infection with human rhinovirus serotype 16. 5-LO-positive cell counts increased 9-fold (from 0.48 to 4.4 cells/mm(2); P.05), and 5-LO-activating protein (FLAP)-positive cell counts increased 3.6-fold (from 1.8 to 6.5 cells/mm(2); P =.09). Levels of leukotriene A(4) hydrolase and leukotriene C(4) synthase were unchanged. COX-2--positive cell counts increased from 0 to 2.6 cells/mm(2) (P =.009), with no change in COX-1 levels. Increases of 3-4-fold were seen in levels of macrophages (P =.02) and mast cells (P =.07) but not of eosinophils (P.4), and bronchoalveolar lavage fluid cysteinyl-leukotriene levels doubled (from 11.2 to 20.4 pg/mL; P =.13). Cold symptom scores correlated with bronchial immunostaining for FLAP (rho = 0.93; P =.001). In normal subjects, rhinovirus colds induce bronchial inflammation with markedly enhanced expression of 5-LO pathway proteins and COX-2. |
| Databáze: | OpenAIRE |
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