Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways
| Autor: | Jinge Kong, Shuying Dong, Wenbing Sun, Jun Gao, Xuemei Ding, Shan Ke, Fandong Kong, Shaohong Wang, Lemin Zheng, Jian Kong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Oncology
medicine.medical_specialty Carcinoma Hepatocellular Epithelial-Mesenchymal Transition Radiofrequency ablation Hepatocellular carcinoma MAP Kinase Signaling System medicine.medical_treatment Morpholines Erk signaling Mice Nude Catheter ablation General Biochemistry Genetics and Molecular Biology law.invention Metastasis Mice law Internal medicine Cell Line Tumor medicine Animals Humans Epithelial–mesenchymal transition Neoplasm Metastasis Protein kinase B Medicine(all) Flavonoids business.industry Biochemistry Genetics and Molecular Biology(all) Research Liver Neoplasms General Medicine medicine.disease digestive system diseases Insufficient radiofrequency ablation Tumor progression Chromones Catheter Ablation Disease Progression business Proto-Oncogene Proteins c-akt |
| Zdroj: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Popis: | Background Residual tumor progression after insufficient radiofrequency ablation (RFA) has been recently reported. However, whether epithelial-mesenchymal transition (EMT), which is a key process that drives cancer metastasis, is involved in the tumor progression after insufficient RFA is not well understood. Methods Human hepatocellular carcinoma (HCC) cell lines SMMC7721 and Huh7 were used. Insufficient RFA was simulated using a water bath (47°C 5 min, 10 min, 15 min, 20 min and 25 min gradually). MTT assay was used to evaluate the proliferation of HCC cells in vitro. Migration and invasion of HCC cells were determined by transwell assay. The molecular changes in HCC cells after insufficient RFA were evaluated by western blot. LY294002 and PD98059 were used to treat HCC cells. An ectopic nude mice model and a tail vein metastatic assay were used to evaluate the growth and metastatic potential of SMMC7721 cells in vivo after insufficient RFA. Results SMMC7721 and Huh7 cells after insufficient RFA (named as SMMC7721-H and Huh7-H respectively) exhibited enhanced proliferation, migration and invasion (6.4% and 23.6%, 33.2% and 66.1%, and 44.1% and 57.4% increase respectively) in vitro. Molecular changes of EMT were observed in SMMC7721-H and Huh7-H cells. LY294002 and PD98059 inhibited the EMT of SMMC7721-H and Huh7-H cells. SMMC7721-H cells also exhibited larger tumor size (1440.8 ± 250.3 mm3 versus 1048.56 ± 227.6 mm3) and more lung metastasis (97.4% increase) than SMMC7721 cells in vivo. Higher expression of PCNA, N-cadherin and MMP-2 and MMP-9, was also observed in SMMC7721-H tumors. Conclusions Insufficient RFA could directly promote the invasiveness and metastasis of HCC cells. Insufficient RFA may promote the EMT of HCC cells through Akt and ERK signaling pathways. |
| Databáze: | OpenAIRE |
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