Activity of plant extracts on the respiratory burst and the stress protein synthesis
| Autor: | Tomás Adzet, Esther Marín, Francisco Pérez-García, Salvador Cañigueral |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Neutrophils
Pharmaceutical Science HSP72 Heat-Shock Proteins Asteraceae medicine.disease_cause law.invention chemistry.chemical_compound law Drug Discovery medicine Humans Hydrogen peroxide Medicine African Traditional Heat-Shock Proteins Reactive nitrogen species Respiratory Burst Pharmacology chemistry.chemical_classification Reactive oxygen species Plants Medicinal biology Plant Extracts Baccharis Flow Cytometry biology.organism_classification Respiratory burst Complementary and alternative medicine chemistry Biochemistry Molecular Medicine Sodium nitroprusside Reactive Oxygen Species Phytotherapy Oxidative stress medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0944-7113 |
| Popis: | Aqueous, methanol and dichloromethane extracts from Artemisia copa, Baccharis grisebachii, Baccharis incarum, Baccharis latifolia, Mutisia kurtzii and Pluchea sagittalis, plants used in the Traditional Medicine of South America, are studied for activity on the respiratory burst and the inducible heat shock protein of 72 kD (hsp72) synthesis. Activity on the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS), as well as on hsp72 synthesis was measured by flow cytometry in human neutrophils. Cells were stimulated using hydrogen peroxide, phorbol-12-myristate-13-acetate (PMA) or formyl-methionyl-leucyl-phenylalanine (FMLP) for ROS generation, and sodium nitroprusside (SNP) or PMA in the presence of calmodulin inhibitor W-13 for RNS. The production of hsp72 was induced by heat, PMA, H2O2 and SNP. The best inhibitory activity was shown by the dichloromethane extracts of Baccharis grisebachii and Pluchea sagittalis that were active in all the assays. The aqueous extract of Pluchea sagittalis was also active in most assays. The aqueous extract from Mutisia kurtzii caused a clear increase of the hsp72 production and showed prooxidant activity. |
| Databáze: | OpenAIRE |
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