Macrophages mediate corticotomy-accelerated orthodontic tooth movement
| Autor: | Wen Sun, Zongyang Sun, Chen Yali, Shuting Zhang, Yan Wang, Yan Bin, Linlin Zhu, Hanwen Zhang, Lizhe Xie, Siyu Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
STAT3 Transcription Factor
0301 basic medicine Tooth Movement Techniques Osteoimmunology Macrophage polarization lcsh:Medicine Article Stat3 Signaling Pathway Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine Orthodontic Appliances Osteogenesis Alveolar Process medicine Animals lcsh:Science Dental alveolus Janus Kinases Multidisciplinary Tumor Necrosis Factor-alpha Chemistry Macrophages Monocyte lcsh:R NF-kappa B 030206 dentistry Rats Cell biology Phenotype 030104 developmental biology medicine.anatomical_structure lcsh:Q Signal transduction Signal Transduction Corticotomy |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Clinical evidence has suggested that surgical corticotomy of the alveolar bone can accelerate local orthodontic tooth movement (OTM), but the underlying cell and molecular mechanisms remain largely unclear. The present study examined the role of macrophages played in corticotomy-assisted OTM. Orthodontic nickel-titanium springs were applied to the left maxillary first molars of rats or mice to induce OTM with or without corticotomy. Corticotomy enhanced OTM distance by accelerating movement through induction of local osteoclastogenesis and macrophage infiltration during OTM. Further analysis showed that macrophages were polarized toward an M1-like phenotype immediately after corticotomy and then switched to an M2-like phenotype during OTM. The microenvironment of corticotomy induced macrophage infiltration and polarization through the production of TNF-α. More importantly, the amount of OTM induced by corticotomy was significantly decreased after mice were depleted of monocyte/macrophages by injection of liposome-encapsulated clodronate. Further experiments by incubating cultured macrophages with fresh tissue suspension obtained from post-corticotomy gingiva switched the cells to an M1 phenotype through activation of the nuclear factor-κB (NF-κB) signaling pathway, and to an M2 phenotype through activation of the JAK/STAT3 signaling pathway. Our results suggest that corticotomy induces macrophage polarization first by activating the NF-κB signaling pathway and later by activating the JAK/STAT3 signaling pathway, and that these processes contribute to OTM by triggering production of inflammatory cytokines and osteoclastogenesis. |
| Databáze: | OpenAIRE |
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