Prostaglandin biosynthesis and stimulation of cyclic AMP in primary monolayer cultures of epithelial cells from mouse mammary gland
| Autor: | David V. Maudsley, Sheila A. Hunter, Sumner H. Burstein, George Gagnon |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
medicine.medical_specialty
Naproxen Period (gene) medicine.medical_treatment Stimulation Mice Inbred Strains Biology Biochemistry Basal (phylogenetics) Mice Endocrinology Mammary Glands Animal Theophylline Pregnancy Internal medicine medicine Cyclic AMP Animals Drug Interactions Prostaglandins E Prostaglandins F Phosphodiesterase Radioimmunoassay Female Collagen medicine.drug Prostaglandin E |
| Zdroj: | Prostaglandins. 11(1) |
| ISSN: | 0090-6980 |
| Popis: | Cyclic AMP levels in primary monolayer cultures of epithelial cells prepared from mid-pregnant mice are stimulated by prostaglandin E 1 and E 2 . Prostaglandin F 1α and F 2α have only a slight effect upon cyclic AMP levels. In the absence of phosphodiesterase inhibitors the rise in cyclic AMP produced by PGE 1 is only transient and the levels return to normal within 30 minutes. High concentrations (16 mM) of theophylline are needed to prevent this decline, suggesting that the phosphodiesterase activity of epithelial cells in culture is high. However, theophylline alone produced only a small increase in basal cyclic AMP levels even over a 2-hour period indicating that basal cyclic AMP is turned over more slowly than cyclic AMP produced in response to stimulation with PGE 1 . Both PGE and PGF synthesis were monitored using radioimmunoassay procedures previously reported. The observed levels were found to decrease as cell density increased and were sensitive to the addition of agents such as collagen and naproxen. |
| Databáze: | OpenAIRE |
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