Circulating lncRNA DANCR as a potential auxillary biomarker for the diagnosis and prognostic prediction of colorectal cancer

Autor: Xianjuan Shen, Xudong Wang, Yajing Xue, Zhiwei Fan, Xiaopeng Cui, Shaoqing Ju, Hui Cong
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Oncology
Colorectal cancer
Prognostic prediction
Biochemistry
0302 clinical medicine
Databases
Genetic
auxiliary diagnosis
Diagnostics & Biomarkers
Research Articles
Cancer
Area under the curve
Middle Aged
Prognosis
Gene Expression Regulation
Neoplastic
Real-time polymerase chain reaction
Area Under Curve
030220 oncology & carcinogenesis
Biomarker (medicine)
Female
RNA
Long Noncoding
Colorectal Neoplasms
Cell-Free Nucleic Acids
Adult
China
medicine.medical_specialty
Biophysics
colorectal cancer
03 medical and health sciences
Internal medicine
Biomarkers
Tumor
medicine
Humans
neoplasms
Molecular Biology
Aged
Microarray analysis techniques
business.industry
Cell Biology
medicine.disease
digestive system diseases
030104 developmental biology
ROC Curve
Colorectal Polyp
RNA
DANCR
real-time PCR
business
Zdroj: Bioscience Reports
ISSN: 1573-4935
0144-8463
Popis: Studies have shown that long non-coding RNAs (lncRNAs) play vital roles in the development of cancer, including colorectal cancer (CRC). Our purpose is to validate the diagnostic value of serum differentiation antagonizing non-protein coding RNA (DANCR) in CRC by focusing on its expression and clinical application. lncRNA expression profiles of CRC patients were obtained and analyzed by repurposing the publically available microarray data. Tissue or serum specimens were obtained from 40 patients with primary CRC, 10 patients with recurrent CRC, 40 patients with colorectal polyps, and 40 healthy controls. It was found that DANCR level in the CRC tissue and serum was significantly increased, and serum DANCR expression was decreased in post-operative patients as compared with that in pre-treatment patients and recurrent patients. In addition, serum DANCR expression was significantly correlated with different TNM stages. Correlation analysis of DANCR and other diagnostic indicators showed that the serum DANCR expression level was significantly correlated with CA199 but not with CEA in CRC patients. As for diagnostic efficiency by ROC analysis, the area under the curve (AUC) of serum DANCR was higher than that of CEA and CA199 in CRC group vs. colorectal polyp group. Simultaneous detection of DANCR, CEA and CA199 yielded the highest sensitivity and AUC as compared with either of them alone. Taken together, serum DANCR was up-regulated in CRC patients and high expression of DANCR may prove to be a potential biomarker for the diagnosis of CRC.
Databáze: OpenAIRE
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