QTL analysis coupled with PTR-ToF-MS and candidate gene-based association mapping validate the role of Md-AAT1 as a major gene in the control of flavor in apple fruit
| Autor: | Fabrizio Costa, Iuliia Khomenko, Andrea Romano, Mario Di Guardo, Guglielmo Costa, Riccardo Velasco, Luca Cappellin, Nicola Busatto, Franco Biasioli, Brian Farneti |
|---|---|
| Přispěvatelé: | L. Cappellin, B. Farneti, M. D. Guardo, N. Busatto, I. Khomenko, A. Romano, R. Velasco, G. Costa, F. Biasioli, F. Costa |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Candidate gene
Apple flavor Candidate gene-based association mapping Fruit quality QTL analysis Volatile organic compounds Molecular Biology Plant Science QTL analysis Apple flavor Population Computational biology Plant Science Quantitative trait locus Biology Metabolomics Volatile organic compounds Allele Association mapping education Gene Molecular Biology education.field_of_study Fruit quality Apple flavour business.industry Candidate gene-based association mapping Apple food and beverages QTL analysi Volatile organic compound Major gene Biotechnology Settore AGR/03 - ARBORICOLTURA GENERALE E COLTIVAZIONI ARBOREE business |
| Popis: | Volatile organic compounds (VOCs) are fundamental elements of flavor, one of the most important fruit-quality traits. Despite its importance, this aspect is still poorly considered in assisted breeding programs, due to the lack of suitable and fast detection systems as well as validated functional markers. In this work, a full-sib parental mapping population (‘Fuji × Delearly’) was initially employed to perform a comprehensive quantitative trait locus (QTL) survey, to assess the VOC segregation detected by a novel proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS) on fruit collected after a 2-month period of postharvest storage. Among this set of genomic regions, on chromosome 2 was also verified the coincident location between a group of QTLs, mainly associated to esters and alcohols, with a functional marker designed for Md-AAT1, a gene involved in the last step of the ester biosynthetic pathway. The allelic effect of this marker (here named Md-AAT1SSR) was further validated by candidate gene association mapping approach in a collection of 124 apple accessions. In this case, the volatile profiling was performed on peeled fruit flesh, as an important fraction of the aromatic blend of apple is released only after cutting. This work proposed a new and fast method for aroma phenotyping as well as a novel marker for an easy and widely applicable apple fruit quality advanced selection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|