Development of a sensitive and specific quantitative reverse transcription-polymerase chain reaction assay for blood thyroglobulin messenger ribonucleic acid in the follow-up of patients with differentiated thyroid carcinoma
| Autor: | Valter Tadeu Boldarine, Jairo Tabacow Hidal, Danielle M. Andreoni, Maria Conceição Mamone, Gustavo S. Guimarães, Rosa Paula M. Biscolla, Teresa S. Kasamatsu, Elza S. Ikejiri, Felipe Crispim, Cleber P. Camacho, Rui M. B. Maciel, Cláudia C. D. Nakabashi, Flávio Carneiro Hojaij |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male medicine.medical_specialty DNA Complementary Adolescent Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Context (language use) Biochemistry Thyroglobulin Thyroid carcinoma Young Adult Endocrinology Internal medicine medicine Humans RNA Messenger Thyroid Neoplasms Neoplasm Metastasis Thyroid cancer Tumor marker Aged DNA Primers Ultrasonography Messenger RNA business.industry Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) Thyroid Middle Aged medicine.disease Long-Term Care Reverse transcription polymerase chain reaction medicine.anatomical_structure ROC Curve Female Neoplasm Recurrence Local business Neck |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 95(4) |
| ISSN: | 1945-7197 |
| Popis: | Serum thyroglobulin is a sensitive tumor marker in the follow-up of patients with differentiated thyroid carcinoma (DTC), but the presence of endogenous anti-thyroglobulin antibodies (TgAb) can interfere on its measurement. To prevent interference by TgAb, several investigators have tried to quantify blood mRNA Tg by real-time RT-PCR, but the results have been variable, not reporting a correlation between mRNA Tg and the presence of metastases.The aim of the study was to evaluate the development of a sensitive and specific quantitative RT-PCR assay for blood mRNA Tg in the follow-up of patients with DTC.An assay employing primers located in a region not affected by alternative splicing or single nucleotide polymorphisms was developed to study 104 DTC patients (13 of 104 with positive TgAb).The assay is specific for thyroid tissue because we found mRNA Tg expression in normal thyroid tissue, but we did not find any mRNA Tg expression in any extrathyroidal tissues. Quantitative mRNA Tg levels were significantly different between patients "free of disease" (82 of 104) and those with metastases (22 of 104) (2.61 +/- 0.26 vs. 27.58 +/- 1.62 pg mRNA Tg/microg RNA) (P0.0001). A cutoff point of 5.51 was able to discriminate between the two groups. In addition, the measurement of mRNA Tg was not affected by the presence of TgAb.This new mRNA Tg quantification is a reliable method that allowed us to differentiate patients free of disease from those with metastases, and it could represent an appropriate molecular marker for the follow-up of patients with DTC, especially those with positive TgAb. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|