Developmentally regulated expression of the PD-1 protein on the surface of double-negative(CD4–CD8–) thymocytes
| Autor: | Yasutoshi Agata, Tasuku Honjo, Sadao Imamura, Nagahiro Minato, Akemi Kawasaki, Masaki Sato, Hiroyuki Nishimura, Hideo Yagita, Toru Nakano |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Aging
CD3 Complex CD8 Antigens T cell Programmed Cell Death 1 Receptor Immunology Double negative Thymus Gland Lymphocyte Activation Mice T-Lymphocyte Subsets medicine Animals Immunology and Allergy IL-2 receptor biology T-cell receptor CD44 Antibodies Monoclonal Proteins Cell Differentiation Receptors Antigen T-Cell gamma-delta Receptors Interleukin-2 General Medicine Molecular biology Neoplasm Proteins DNA-Binding Proteins Mice Inbred C57BL Hyaluronan Receptors medicine.anatomical_structure Suppression subtractive hybridization Antigens Surface CD4 Antigens biology.protein Female Antibody Apoptosis Regulatory Proteins CD8 |
| Zdroj: | International Immunology. 8:773-780 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/8.5.773 |
| Popis: | PD-1, a member of the Ig superfamily, was previously isolated from an apoptosis-induced T cell hybridoma 2B4.11 by subtractive hybridization. Expresson of the PD-1 mRNA is restricted to thymus in adult mice. Using an anti-PD-1 mAb (J43), we examined expression of the PD-1 protein during differentiation of thymocytes in normal adult, fetal and RAG-2(-/-) mice with or without anti-CD3 mAb stimulation. While PD-1 was expressed only on 3-5% of total normal thymocytes, approximately 34% of the CD4(-)CD8(-) double-negative (DN) fraction are PD-1(+) cells with two distinct expression levels (low and high). PD-1(high) thymocytes belonged to TCR gammadelta lineage cells. In the DN compartment of the TCR alphabeta lineage, PD-1 expression started at the low level from the CD44(+)CD25(+) stage and the majority of thymocytes expressed PD-1 at the CD44(-)CD25(-) stage in which the thymocytes express TCR beta chains. The anti-CD3epsilon antibody administration augmented the PD-1 expression as well as the differentiation of the CD44(-)CD25(+) DN cells into the CD44(-)CD25(-) DN stage, not only in normal mice but also in RAG-2-deficient mice. The fraction of the PD-1(low) cells in the CD4(+)CD8(+) double-positive (DP) compartment was very small ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|