Antisense oligonucleotide targeting midkine suppresses in vivo angiogenesis
| Autor: | Jian-fang He, Li-Cheng Dai, Yong-Liang Lu, Xing Yao, Jin-liang Ping, Xiang Wang |
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| Rok vydání: | 2007 |
| Předmět: |
In situ
animal structures Carcinoma Hepatocellular Angiogenesis Chick Embryo Biology Umbilical vein Cell Line Oligodeoxyribonucleotides Antisense In vivo medicine Animals Humans Midkine Neovascularization Pathologic Cell growth Liver Neoplasms Gastroenterology General Medicine medicine.disease Virology Xenograft Model Antitumor Assays digestive system diseases Staining Basic Research Hepatocellular carcinoma Cancer research biology.protein Cytokines |
| Zdroj: | World journal of gastroenterology. 13(8) |
| ISSN: | 1007-9327 |
| Popis: | To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC).An in situ human hepatocellular carcinoma (HCC) model and CAM assay were used in this experiment. The effect of MK-AS on angiogenesis was evaluated by cell proliferation assay and hematoxylin-eosin (HE) staining.MK-AS significantly inhibited human umbilical vein endothelial cells (HUVEC) and in situ human HCC growth. At the same time, MK-AS suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced CAM and in situ human HCC tissues.MK-AS is an effective antiangiogenesis agent in vivo. |
| Databáze: | OpenAIRE |
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