Progressive Increase in Telomerase Activity From Benign Melanocytic Conditions to Malignant Melanoma
| Autor: | Elena Pagani, P. M. Lacal, S. D'Atri, R. S. Taylor, T. Faraggiana, Ruben D. Ramirez, Jerry W. Shay |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
Telomerase Pathology medicine.medical_specialty Skin Neoplasms Mitosis In situ hybridization Biology lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine medicine Humans Neoplasm Invasiveness metastases Lymph node Melanoma In Situ Hybridization 030304 developmental biology 0303 health sciences Nevus Pigmented immortality skin cancer RNA medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens telomeres Telomere medicine.anatomical_structure 030220 oncology & carcinogenesis Lymph prognosis Skin cancer Research Article |
| Zdroj: | Neoplasia: An International Journal for Oncology Research, Vol 1, Iss 1, Pp 42-49 (1999) |
| ISSN: | 1476-5586 |
| DOI: | 10.1038/sj.neo.7900004 |
| Popis: | The expression of telomerase activity and the in situ localization of the human telomerase RNA component (hTR) in melanocytic skin lesions was evaluated in specimens from sixty-three patients. Specimens of melanocytic nevi, primary melanomas and subcutaneous metastases of melanoma were obtained from fifty-eight patients, whereas metastasized lymph nodes were obtained from five patients. Telomerase activity was determined in these specimens by using a Polymerase Chain Reaction—based assay (TRAP). High relative mean telomerase activity levels were detected in metastatic melanoma (subcutaneous metastasess = 54.5, lymph node metastasess = 56.5). Much lower levels were detected in primary melanomas, which increased with advancing levels of tumor cell penetration (Clark II = 0.02, Clark III = 1.1, and Clark IV = 1.9). Twenty-six formalin-fixed, paraffin-embedded melanocytic lesions were sectioned and analyzed for telomerase RNA with a radioactive in situ hybridization assay. In situ hybridization studies with a probe to the template RNA component of telomerase confirmed that expression was almost exclusively confined to tumor cells and not infiltrating lymphocytes. These results indicate that levels of telomerase activity and telomerase RNA in melanocytic lesions correlate well with clinical stage and could potentially assist in the diagnosis of borderline lesions. |
| Databáze: | OpenAIRE |
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