Gene expression regulation following behavioral sensitization to cocaine in transgenic mice lacking the glucocorticoid receptor in the brain
| Autor: | M. Le Moal, Mohamed Jaber, Véronique Deroche-Gamonet, R. Izawa, Christoph Kellendonk, I. Sillaber, Pier Vincenzo Piazza, François Tronche |
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| Přispěvatelé: | Institut de physiologie et biologie cellulaires (IPBC), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire, neurophysiologie et comportement (GMNC), Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2006 |
| Předmět: |
Male
comportement Dynorphin Substance P Synaptic Transmission Mice 0302 clinical medicine Mineralocorticoid receptor Glucocorticoid receptor Cocaine Dopamine Uptake Inhibitors Receptors Kainic Acid neuromediateurs neurone In Situ Hybridization PASCAL 0303 health sciences Kainic Acid Behavior Animal General Neuroscience Enkephalins 3. Good health Dopamine receptor NMDA receptor dépendance hormones hormone substitutes and hormone antagonists expression des gènes medicine.medical_specialty Mice Transgenic [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Dynorphins Receptors N-Methyl-D-Aspartate 03 medical and health sciences Receptors Glucocorticoid Internal medicine Dopamine receptor D2 medicine Animals ACTH receptor RNA Messenger 030304 developmental biology Brain Chemistry Sigma-1 receptor Receptors Dopamine D2 Receptors Dopamine D1 gène neurosciences adaptation au stress Endocrinology Gene Expression Regulation 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Neuroscience, Elsevier-International Brain Research Organization, 2006, 137, pp.915-924 |
| ISSN: | 0306-4522 1873-7544 |
| Popis: | Several findings suggest that glucocorticoid hormones influence the propensity of an individual to develop cocaine abuse. These hormones activate two related transcription factors, the glucocorticoid receptor and the mineralocorticoid receptor. We have shown previously that mice carrying a mutation of the glucocorticoid receptor gene specifically in neural cells, glucocorticoid receptor knock-out in the brain, show a dramatic decrease in cocaine-induced self-administration and no behavioral sensitization to this drug, two experimental procedures considered relevant models of addiction. Here, we investigated in glucocorticoid receptor knock-out in the brain mice the consequences of this mutation at the level of the expression of neuropeptide, dopamine receptor and glutamate receptor subunit mRNAs. We quantified mRNA levels in the cortex, striatum and accumbens under basal conditions and following acute or repeated cocaine treatments. Our results show that, under basal conditions, neuropeptide (substance P, dynorphin) and dopamine receptor (D1, D2) mRNAs were decreased in glucocorticoid receptor knock-out in the brain mice in the dorsal striatum but not in the accumbens. However, cocaine-induced changes in the levels of these mRNAs were not modified in glucocorticoid receptor knock-out in the brain mice. In contrast, mutant mice showed altered response in mRNA levels of N-methyl-D-aspartate, GLUR5 and GLUR6 glutamate receptor subunits as well as of enkephalin following cocaine administration. These modifications may be associated to decrease of behavioral effects of cocaine observed in glucocorticoid receptor knock-out in the brain mice. |
| Databáze: | OpenAIRE |
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