On-Bead Quantitation of Resin Bound Functional Groups Using Analogue Techniques with Vibrational Spectroscopy

Autor:	Don E. Pivonka
Rok vydání:	1999
Předmět:	Quantitative Biology::Biomolecules Infrared Ligand Chemistry Analytical chemistry Infrared spectroscopy General Chemistry Spectral line Condensed Matter::Soft Condensed Matter Solvent chemistry.chemical_compound symbols.namesake Solid-phase synthesis Functional group symbols Raman spectroscopy
Zdroj:	Journal of Combinatorial Chemistry. 2:33-38
ISSN:	1520-4774 1520-4766
DOI:	10.1021/cc9900403
Popis:	This report introduces two novel methodologies for the on-bead, functional group quantitation of solid phase synthesis using vibrational spectroscopy. "Analogue bleed" quantitation is an infrared spectroscopic technique in which combinatorial library bead spectral data are regressed against solution phase functional analogue spectra of a ligand in question. "Dual Analogue" quantitation is introduced as an infrared or Raman technique in which ligand-to-styrene band ratios of solution phase ligand/styrene monomer standards are used to generate an internally path length referenced calibration which can then be applied to solvent swollen beads. These technologies have application across a wide range of functional groups and can be applied throughout each step of a resin bound synthesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9bcda8a9c3e6242bde9ebd527d7cb2d https://doi.org/10.1021/cc9900403 Zobrazit plný text záznamu