Human HOX genes are differentially activated by retinoic acid in embryonal carcinoma cells according to their position within the four loci
| Autor: | Maria Pannese, Antonio Simeone, Antonio Faiella, Dario Acampora, Marco Rambaldi, Maurizio D'Esposito, Edoardo Boncinelli, Enrica Migliaccio, Anna Stornaiuolo, Vincenzo Nigro, Franco Morelli |
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| Přispěvatelé: | Stornaiuolo, A, Acampora, D, Pannese, M, D'Esposito, M, Morelli, F, Migliaccio, E, Rambaldi, M, Faiella, A, Nigro, Vincenzo, Simeone, A. |
| Rok vydání: | 1990 |
| Předmět: |
Embryonal Carcinoma Stem Cells
Molecular Sequence Data Restriction Mapping Retinoic acid Tretinoin Biology chemistry.chemical_compound Sequence Homology Nucleic Acid medicine Tumor Cells Cultured Animals Humans Amino Acid Sequence Hox gene Base Sequence Genes Homeobox Blotting Northern Molecular biology Retinoic acid receptor P19 cell chemistry Gene Expression Regulation Genes Neoplastic Stem Cells Homeobox Homeotic gene Developmental Biology medicine.drug |
| Zdroj: | Cell differentiation and development : the official journal of the International Society of Developmental Biologists. 31(2) |
| ISSN: | 0922-3371 |
| Popis: | We studied the expression of 33 human homeobox genes belonging to four complex HOX loci in embryonal carcinoma NT2/D1 cells. These cells can be induced to differentiate by culturing them in media containing retinoic acid. Northern blot analysis reveals that no expression of these genes was detectable in NT2/D1 stem cells, whereas 22 HOX genes are well expressed in NT2/D1 cells treated with 10 microM retinoic acid for 14 days. The 11 HOX genes the expression of which remained undetectable in NT2/D1 cells after this treatment are located at the 5' end of their loci: four in HOX1, five in HOX3 and two in HOX4. The boundary between induced and silent genes roughly corresponds to the HOX genes constituting the homology group 5, related to the Abdominal-B homeotic gene of Drosophila. All nine identified HOX2 genes are well expressed in fully induced NT2/D1 cells and none of them maps 5' genes of this homology group. We conclude that HOX genes are differentially activated by retinoic acid in these cells according to their physical location within the four chromosomal loci. |
| Databáze: | OpenAIRE |
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