Proteomic analysis of plasma from patients during an acute coronary syndrome
| Autor: | Sergio Alonso-Orgaz, Antonio López Farré, Petra J. Mateos-Cáceres, Carlos Macaya, Antonio Núñez, Marta Escribano Burgos, Juan J. Granizo, Raimundo de Andrés, Juan Gómez, Antonio García-Méndez, Carolina Carrasco, L. Rico, Jerónimo Farré |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Proteomics Acute coronary syndrome medicine.medical_specialty Myocardial Infarction Angina Reference Values Risk Factors Internal medicine Blood plasma Humans Protein Isoforms Medicine Angina Unstable Aged Apolipoprotein A-I business.industry Fibrinogen Blood Proteins Syndrome Middle Aged medicine.disease Blood proteins Coronary heart disease Endocrinology alpha 1-Antitrypsin Reference values Acute Disease Cardiology Female Cardiology and Cardiovascular Medicine business |
| Zdroj: | Journal of the American College of Cardiology. 44:1578-1583 |
| ISSN: | 0735-1097 |
| DOI: | 10.1016/j.jacc.2004.06.073 |
| Popis: | ObjectivesThe aim of this study was to analyze modifications in the plasma protein map during an acute coronary syndrome (ACS) using proteomics.BackgroundProteomics is a new technology that allows the detection and identification of several proteins at a given time in a sample.MethodsPlasma from 19 patients, 11 with acute myocardial infarction (AMI) and 8 with unstable angina (UA), was investigated. The control group included nine age-matched volunteers.ResultsIn two-dimensional electrophoresis using a pH range of 4 to 7, constant differences were found in at least four different areas within the plasma protein map. In area 1, we identified the presence of seven alpha1-antitrypsin (AAT) isoforms in plasma from control subjects. alpha1-antitrypsin isoform 1 was undetectable in plasma from UA and AMI patients. The AAT isoforms 5, 6, and 7 were reduced in plasma from AMI patients when compared with UA patients. Three fibrinogen gamma chain isoforms were identified in area 2. Fibrinogen gamma chain isoforms 1 and 2 were increased in AMI patients with respect to UA patients. Five apolipoprotein A-I isoforms were identified in area 3. All of them were reduced in plasma from AMI patients with respect to UA patients. In area 4, the γ-immunoglobulin heavy chains were detected and were found increased in plasma from ACS patients.ConclusionsPlasma proteomic analysis makes it possible to develop a map of the protein isoforms that are expressed in plasma during an ACS. |
| Databáze: | OpenAIRE |
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