Halomonas shantousis sp. nov., a novel biogenic amines degrading bacterium isolated from Chinese fermented fish sauce
| Autor: | Ying Xu, Jinzhi Yu, Binghong Xu, Ning Ma, Dongfeng Wang, Xiaoyan Dong, Chunsheng Li, Wei Jiang |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Biogenic Amines China Sequence analysis Molecular Sequence Data Sodium Chloride Microbiology DNA Ribosomal Cytosol RNA Ribosomal 16S Animals Cluster Analysis Molecular Biology Biotransformation Phylogeny Fermented fish Halomonas Base Composition biology Strain (chemistry) Fatty Acids Quinones Temperature Nucleic Acid Hybridization General Medicine Sequence Analysis DNA Ribosomal RNA Hydrogen-Ion Concentration biology.organism_classification 16S ribosomal RNA Halophile Aerobiosis Bacterial Typing Techniques Food Microbiology Bacteria Locomotion |
| Zdroj: | Antonie van Leeuwenhoek. 106(6) |
| ISSN: | 1572-9699 |
| Popis: | A Gram-negative, aerobic, short rod-shaped and non-motile bacterium, designated SWA25(T), was isolated from Chinese fermented fish sauce in Shantou, Guangdong Province, China. Strain SWA25(T) was moderately halophilic, formed colourless colonies and grew at 10-45 °C (optimum, 37 °C) and pH 4-9 (optimum, 6-7) in the presence of 0.5-22.5 % (w/v) NaCl (optimum, 3 %). The major cellular fatty acids (>10 %) were identified as C18:1 ω7C, C16:0, C16:1 ω7c, and C19:0 cyclo ω8c, and the predominant respiratory ubiquinone was Q-9. The genomic DNA G+C content was 61.3 ± 2.1 mol %. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SWA25(T) belonged to the genus Halomonas in the family Halomonadaceae. The closest relatives were Halomonas xianhensis A-1(T) (96.5 % 16S rRNA gene sequence similarity), H. lutea DSM 23508(T) (96.5 %) and H. muralis LMG 20969(T) (96.1 %). DNA-DNA hybridization assays showed 30.7 ± 2.6 % relatedness between strain SWA25(T) and H. xianhensis A-1(T), and 39.4 ± 4.1 % between strain SWA25(T) and H. lutea DSM 23508(T). On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain SWA25(T) should be placed in the genus Halomonas as a representative of a novel species. The name Halomonas shantousis sp. nov. is proposed, with SWA25(T)(=CCTCC AB 2013151(T) = JCM 19368(T)) as the type strain. |
| Databáze: | OpenAIRE |
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