Synthesis of dicarboxylic acylcarnitines
| Autor: | David W. Johnson |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Spectrometry Mass Electrospray Ionization Chromatography Magnetic Resonance Spectroscopy Carnitine chloride Chemistry Electrospray ionization Organic Chemistry Cell Biology Nuclear magnetic resonance spectroscopy Tandem mass spectrometry Mass spectrometry Deuterium Biochemistry chemistry.chemical_compound Dicarboxylic acid Carnitine Trifluoroacetic acid Organic chemistry lipids (amino acids peptides and proteins) Dicarboxylic Acids Molecular Biology Beta oxidation |
| Zdroj: | Chemistry and physics of lipids. 129(2) |
| ISSN: | 0009-3084 |
| Popis: | Syntheses of malonyl, methylmalonyl, succinyl, glutaryl, methylglutaryl, dodecanedioyl and hexadecanedioyl carnitines are described. The dicarboxylic acylcarnitines were prepared from eight equivalents of cyclic anhydride or isopropylidene ester of the dicarboxylic acid and carnitine chloride in trifluoroacetic acid solution. Long chain dicarboxylic acylcarnitines were additionally purified by partitioning between water and n-butanol. Stable isotope labeled analogs, containing 3, 6 or 9 deuterium atoms, were also prepared. They are for use as standards in the electrospray ionization tandem mass spectrometric analysis of dicarboxylic acylcarnitines in samples from patients with inherited disorders of fatty acid oxidation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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