Immunoglobulin treatment ameliorates myocardial injury in experimental autoimmune myocarditis associated with suppression of reactive oxygen species
| Autor: | Chiharu Kishimoto, Taka-aki Okabe, Keisuke Shioji, Masaomi Nimata |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Myocarditis Swine medicine.medical_treatment Immunoglobulins Autoimmune Diseases Mice chemistry.chemical_compound Immune system In vivo medicine Animals Humans Cytotoxic T cell chemistry.chemical_classification Mice Inbred BALB C Reactive oxygen species biology business.industry Superoxide medicine.disease Treatment Outcome Cytokine Heart Injuries chemistry Immunology biology.protein Antibody Reactive Oxygen Species Cardiology and Cardiovascular Medicine business |
| Zdroj: | International Journal of Cardiology. 167:140-145 |
| ISSN: | 0167-5273 |
| DOI: | 10.1016/j.ijcard.2011.12.058 |
| Popis: | Aims We tested the hypothesis that immunoglobulin ameliorated experimental autoimmune myocarditis (EAM) in mice attributing to the suppression of reactive oxygen species (ROS)-mediated myocardial injury. Methods We intraperitoneally administered intact type of human immunoglobulin (Ig) or F(ab′) 2 fragments of human immunoglobulin, 1g/kg/day daily for 3weeks, to male BALB/c mice with heart failure due to EAM. Results The results showed that intact type of Ig, but not F(ab′) 2 type, reduced the severity of myocarditis by comparing the heart weight/body weight and lung weight/body weight ratios, pericardial effusion score, macroscopic and microscopic scores. Tissue superoxide production was marked in untreated mice with EAM, which was suppressed by the treatment of immunoglobulins. The cytotoxic activities of lymphocytes in mice with EAM treated with Ig were reduced compared with untreated controls. The shift from Th 1 toward Th 2 cytokine balance was demonstrated by the treatment of immunoglobulins both in vitro and in vivo. Conclusion ROS may be involved in the development of myocarditis. Intact Ig ameliorates myocardial damage in mice with myocarditis associated with suppression of ROS and cytotoxic activity of lymphocytes. |
| Databáze: | OpenAIRE |
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