Studies of human alloantigens on man-mouse hybrids: possible syntheny between hl-a and p systems
| Autor: | C. Billardon, C. Neauport-Sautes, Jean Dausset, P. Couillin, Marc Fellous, Jean Frézal |
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| Rok vydání: | 1973 |
| Předmět: |
Isoantigens
Genotype Immunology Cell Fluorescent Antibody Technique Hybrid Cells Biology Absorption Mice L Cells Antigen Antibody Specificity Histocompatibility Antigens ABO blood group system medicine Animals Humans Immunology and Allergy Trypsin Hybrid Genetics Mice Inbred C3H Immune Sera Complement Fixation Tests Karyotype Fibroblasts Cytotoxicity Tests Immunologic Phenotype Molecular biology Clone Cells medicine.anatomical_structure Karyotyping Blood Group Antigens medicine.drug |
| Zdroj: | European Journal of Immunology. 3:543-548 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830030904 |
| Popis: | The expression of HL-A, P and H (ABO) systems has been studied on man-mouse hybrid clones and their subclones. Using the microcomplement fixation technique, we detected antigens of the HL-A, P and H systems in some clones corresponding to the phenotype of the human cell donor. Absorption tests were used to provide the specificity of the alloantisera. We also found a positive correlation between the presence of HL-A and P1/P antigens in these clones, but no correlation was found between HL-A and Pk specificities and between HL-A and H specificities. It was thought that the positive correlation might be due to a syntheny between HL-A and P loci or to the presence of HL-A and P specificities on a unique molecule. Therefore, preliminary experiments were made using antibody-induced redistribution phenomena (capping) to study the relationships between P and HL-A antigens at the cell surface. They indicated that the two molecules are independent of one another. |
| Databáze: | OpenAIRE |
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