Regulation of quail oviduct phospholipase A2 activity by estradiol
| Autor: | Pageaux Jf, C Laugier, M Lagarde, O Macovschi, S Chanal, Jean-Michel Fayard, B E Felouati |
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| Rok vydání: | 1994 |
| Předmět: |
Gene isoform
medicine.medical_specialty animal structures Endocrinology Diabetes and Metabolism Stimulation Coturnix Cycloheximide Phospholipases A Ornithine decarboxylase chemistry.chemical_compound Endocrinology Phospholipase A2 Internal medicine biology.animal medicine Animals Humans Fallopian Tubes Estradiol biology General Medicine Quail Enzyme Activation Kinetics Phospholipases A2 chemistry Estradiol benzoate biology.protein Oviduct Female Cell Division |
| Zdroj: | European Journal of Endocrinology. 131:205-212 |
| ISSN: | 1479-683X 0804-4643 |
| DOI: | 10.1530/eje.0.1310205 |
| Popis: | Fayard JM, Chanal S, Felouati B, Macovschi O, Lagarde M, Pageaux JF, Laugier C. Regulation of quail oviduct phospholipase A2 activity by estradiol. Eur J Endocrinol 1994;131:205–12. ISSN 0804–4643 The phospholipase A2 (PLA2) activity was measured in the oviduct of immature and estradiol benzoate (EB)-treated quails. The pH profiles demonstrate the presence of two PLA2 isoforms in the avian oviduct: a neutral isoform, optimally active at pH 7–7.5 and calcium independent, responsible for most of the hydrolytic activity in the immature oviduct and poorly stimulated by estradiol; and an alkaline isoform, optimally active at pH 8–9.5 and calcium dependent, with little activity in the immature tissue but markedly stimulated by EB. After EB injection, PLA2 activation occurs at first during the prereplicative period of oviduct cells (+172% at 6 h), it is dose dependent from 0.01 to 1 mg/kg EB and can be prevented by cycloheximide together with ornithine decarboxylase activation. Moreover, estradiol was inactive on cell-free extracts of immature oviducts. These results suggest that EB increases PLA2 activity through gene activation and de novo protein synthesis. The correlation between the early stimulation of PLA2 activity and the proliferation of oviduct cells is discussed. JM Fayard, INSERM U.352, Laboratoire de Physiologie–Pharmacodynamie, INSA 406, 69621 Villeurbanne Cedex, France |
| Databáze: | OpenAIRE |
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