Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome
| Autor: | Malin Fex, Åke Lernmark, Christiane S. Hampe, Mikaela Friedman, Gucci Jijuan Gu Urban, Ulf Landegren, Carina Törn, Masood Kamali-Moghaddam, Ping Ren |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male endocrine system endocrine system diseases Glutamate decarboxylase Medical Biotechnology Proximity ligation assay Antigen-Antibody Complex Stiff-Person Syndrome Biology Article Immune system immune system diseases medicine Humans Medicinsk bioteknik Aged Autoantibodies Immunoassay Type 1 diabetes Multidisciplinary medicine.diagnostic_test Glutamate Decarboxylase Autoantibody nutritional and metabolic diseases Immunology in the medical area Middle Aged medicine.disease Peptide Fragments 3. Good health Case-Control Studies Immunology Biomarker (medicine) Female Stiff person syndrome |
| Zdroj: | Scientific Reports; 5, no 11196 (2015) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS. |
| Databáze: | OpenAIRE |
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