The high level of RANTES in the ectopic milieu recruits macrophages and induces their tolerance in progression of endometriosis
| Autor: | Xiao-Qiu Wang, Ling Wang, Da-Jin Li, Yun Wang, Jing Yu, Xue-Zhen Luo, Ying-Li Shi |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
CCR1 Chemokine Polychlorinated Dibenzodioxins Stromal cell T cell Cell Endometriosis Lipopolysaccharide Receptors Receptors CCR1 Gene Expression Apoptosis Immune tolerance Endometrium Endocrinology Immune Tolerance medicine Ascitic Fluid Humans Chemokine CCL5 Molecular Biology Estradiol U937 cell biology hemic and immune systems HLA-DR Antigens U937 Cells Middle Aged Immunohistochemistry Interleukin-12 Interleukin-10 Interleukin 10 medicine.anatomical_structure Immunology Macrophages Peritoneal Cancer research biology.protein Female B7-2 Antigen Stromal Cells |
| Zdroj: | Journal of Molecular Endocrinology. 45:291-299 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1677/jme-09-0177 |
| Popis: | RANTES (C–C chemokine, regulated on activation, normal T cell expressed and secreted) is involved in progression of endometriosis, but the precise mechanism is understood inadequately. This study is to elucidate the roles of RANTES in macrophage recruitment and tolerance in the endometriotic milieu. The expression of RANTES was analyzed by immunohistochemistry. The cell co-cultures were applied to simulate the endometriotic milieu to investigate the regulation of RANTES secretion and its receptor CCR1 expression. Transwell migration assay was used for chemotaxis of U937 cells (macrophage line) to endometrial stromal cells (ESCs) and/or human pelvic mesothelial cells. The expression of CCR1 was analyzed by RT-PCR and qPCR in transcription and by western blot in translation respectively. Concentrations of RANTES, IL10, and IL12p70 were determined by ELISA. The phenotype of U937 cells and apoptosis of ESCs were analyzed by flow cytometry. We have found that the expression of RANTES is significantly higher in the endometriotic tissue and eutopic endometrium than that of the normal endometrium without endometriosis. The combination of 17β-estradiol and dioxin 2,3,7,8-tetrachlorodibenzo-p-dioxin increases significantly RANTES secretion in the endometriosis-associated cell co-culture which can recruit more macrophages, upregulate CCR1 expression, and induce tolerant phenotype, which inhibits the apoptosis of ESC in the milieu. In conclusion, the higher levels of RANTES in the ectopic milieu facilitate the onset and progression of endometriosis by macrophage recruitment and tolerance that in turn inhibits apoptosis and enhances growth of ESC. |
| Databáze: | OpenAIRE |
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