Clinical application of marrow mesenchymal stem cells for hard tissue repair
| Autor: | Motohiro Hirose, Yoshinori Takakura, Shigeyuki Kitamura, Hajime Ohgushi, Hiroko Machida, Kaori Muraki, Noriko Kotobuki |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Ceramics Tissue Engineering Chemistry Cellular differentiation Mesenchymal stem cell Bone Marrow Cells Cell Differentiation Mesenchymal Stem Cells General Medicine Middle Aged Ascorbic acid Cell biology medicine.anatomical_structure Tissue engineering Osteogenesis Bone cell medicine Aluminum Oxide Humans Bone marrow Stem cell Cell Division Stem cell transplantation for articular cartilage repair Aged |
| Zdroj: | ResearcherID |
| ISSN: | 0513-5796 |
| Popis: | Human marrow mesenchymal stem cells were cultured in a medium containing glycerophosphate, ascorbic acid, and dexamethasone (Dex) on alumina ceramic discs and on tissue culture polystyrene (TCPS) dishes. Cell proliferation followed by osteogenic differentiation was observed to be equal on both culture substrata. The differentiation resulted in the appearance of bone-forming osteoblasts, which fabricated mineralized matrices on these substrata. Stem cells kept at 4 degrees C for 24 h outside a CO2 incubator maintained a viability level of more than 90%. The regenerative cultured bone outside the incubator also maintained high alkaline phosphatase activity for several hours. These results verified that cultured bone fabricated at a cell processing center can be transported to distant hospitals for use in hard tissue repair. To date, the tissue engineered cultured bone formed on alumina ceramics in this environment have already been used in clinical situations, such as total ceramic ankle replacements. |
| Databáze: | OpenAIRE |
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