Avian nephritis virus (ANV) on Brazilian chickens farms: circulating genotypes and intra-genotypic diversity
| Autor: | Rodrigo Martins Soares, Luis Ramiro Luna Espinoza, Fábio Gregori, Laila A. R. Beserra |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Avastrovirus Farms Genotype 040301 veterinary sciences Population Sequence Homology Avian nephritis virus Biology Polymerase Chain Reaction 0403 veterinary science Open Reading Frames 03 medical and health sciences Astroviridae Infections Virology Genetic variation Animals Cluster Analysis education Phylogeny Poultry Diseases Genetics Genetic diversity education.field_of_study Phylogenetic tree GENÉTICA MICROBIANA Genetic Variation Sequence Analysis DNA 04 agricultural and veterinary sciences General Medicine 030104 developmental biology Flock Chickens Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-016-3057-7 |
| Popis: | Avian nephritis virus (ANV), which belongs to the family Astroviridae, is associated with different clinical manifestations (including enteric disorders). Despite being frequently found in the avian industry worldwide, information regarding genetic features of these viruses in Brazil is scarce. Therefore, sixty fecal sample pools (5-6 birds of the same flock), representing 60 poultry farms from six Brazilian States, were screened using an astrovirus-specific hemi-nested-PCR assay targeting the conserved ORF1b gene, followed by nucleotide sequencing of amplified products. PCR and phylogenetic analysis confirmed the detection of 21 positive samples to ANV (35 %). In order to investigate the genetic diversity represented by these viruses, amplification, cloning and phylogenetic analysis of the deduced amino acid sequence of ORF2 gene were attempted. Eight samples were successfully cloned (generating 32 clones in total) and sequenced. Based on phylogenetic analysis of ORF2, sequences defined in this study were classified into three genotypes: genotype 5, which has already been described in birds, and two other novel genotypes, tentatively named genotype 8 and 9, all of which occurred in single or mixed infections. Moreover, high intra-genotypic diversity and co-circulation of distinct strains in a same host population were observed. This study revealed the presence of new strains of ANV in Brazilian poultry and their circulation in commercial chicken flocks. |
| Databáze: | OpenAIRE |
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