Potential of Skin Fibroblasts for Application to Anterior Cruciate Ligament Tissue Engineering
| Autor: | Francine Goulet, Luc Chouinard, Jean Lamontagne, Réjean Cloutier, Anne-Marie Larkin, Anne-Marie Belzil, Pierrot Tremblay, Sheila Laverty, Stéphane Chabaud, Bertrand Lussier |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Anterior cruciate ligament Biomedical Engineering lcsh:Medicine Collagen Type I Tissue engineering In vivo medicine Animals Humans Anterior Cruciate Ligament Cells Cultured Transplantation medicine.diagnostic_test Tissue Engineering business.industry Goats lcsh:R Cell Biology Fibroblasts musculoskeletal system medicine.anatomical_structure Collagen Type III surgical procedures operative Skin biopsy Fibrocartilage Cattle Female Implant business human activities Type I collagen Ex vivo |
| Zdroj: | Cell Transplantation, Vol 20 (2011) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | Fibroblasts isolated from skin and from anterior cruciate ligament (ACL) secrete type I and type III collagens in vivo and in vitro. However, it is much easier and practical to obtain a small skin biopsy than an ACL sample to isolate fibroblasts for tissue engineering applications. Various tissue engineering strategies have been proposed for torn ACL replacement. We report here the results of the implantation of bioengineered ACLs (bACLs), reconstructed in vitro using a type I collagen scaffold, anchored with two porous bone plugs to allow bone–ligament–bone surgical engraftment. The bACLs were seeded with autologous living dermal fibroblasts, and grafted for 6 months in goat knee joints. Histological and ultrastructural observations ex vivo demonstrated a highly organized ligamentous structure, rich in type I collagen fibers and cells. Grafts' vascularization and innervation were observed in all bACLs that were entirely reconstructed in vitro. Organized Sharpey's fibers and fibrocartilage, including chondrocytes, were present at the osseous insertion sites of the grafts. They showed remodeling and matrix synthesis postimplantation. Our tissue engineering approach may eventually provide a new solution to replace torn ACL in humans. |
| Databáze: | OpenAIRE |
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